Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)

Reviews (5)Q&A (1)References (21)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3312] to Glutathione Peroxidase 1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutathione Peroxidase 1 antibody [EPR3312]
    See all Glutathione Peroxidase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3312] to Glutathione Peroxidase 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Common marmoset

  • Immunogen

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 150 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: P07203
    (Peptide available as ab156307)

  • Positive control

    • Human fetal liver, 293T, THP1, and HepG2 whole cell lysate (ab7900); Human kidney tissue; 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab108427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Glutathione Peroxidase 1 peptide (ab156307).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.

    • Target

    Images

    • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
      Lane 3: THP1 cell lysate (20 µg)
      Lane 4: HL60 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab108427 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108427 was shown to recognize Glutathione Peroxidase 1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108427 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + THP-1 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + Human fetal liver at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Immunoprecipitation - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)

      ab108427 (purified) at 1/40 immunoprecipitating glutathione peroxidase 1 in 10 μg THP-1 cell lysate (Lanes 1 and 2, observed at 22 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
      All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : 293T cell lysate
      Lane 3 : THP1 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa

    References (21)

    Publishing research using ab108427? Please let us know so that we can cite the reference in this datasheet.

    ab108427 has been referenced in 21 publications.

    • Koeberle SC  et al. Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-?B-driven inflammation through redox-active mechanisms. Redox Biol 28:101388 (2020). PubMed: 31765890
    • Tremblay-Laganière C  et al. Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function. Environ Sci Pollut Res Int 26:2375-2386 (2019). PubMed: 30467749
    • Shao Y  et al. Proteome changes in methylmercury-exposed mouse primary cerebellar granule neurons and astrocytes. Toxicol In Vitro 57:96-104 (2019). PubMed: 30776503
    • Dhanjal NI  et al. Selenium-rich maize modulates the expression of prostaglandin genes in lipopolysaccharide-stimulated RAW264.7 macrophages. Food Funct 10:2839-2846 (2019). PubMed: 31062009
    • Akahoshi N  et al. Dietary selenium deficiency or selenomethionine excess drastically alters organ selenium contents without altering the expression of most selenoproteins in mice. J Nutr Biochem 69:120-129 (2019). PubMed: 31078905
    View all Publications for this product

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Glutathione Peroxidase 1 antibody [EPR3312]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (whole body)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Specification
    whole body
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Erik Schoenmakers

    Verified customer

    Submitted Mar 29 2018

    Western blot abreview for Anti-Glutathione Peroxidase 1 antibody [EPR3312]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Gonad)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    75 µg
    Specification
    Gonad
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 11 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutathione Peroxidase 1 antibody [EPR3312]

     Good
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Ovary)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2015

    Western blot abreview for Anti-Glutathione Peroxidase 1 antibody [EPR3312]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Marmoset (common) Tissue lysate - whole (brain)
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2014

    Western blot abreview for Anti-Glutathione Peroxidase 1 antibody [EPR3312]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Tissue lysate - whole (aorta)
    Specification
    aorta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2013

    Question

    Vielen Dank fuer Ihre Email.

    Ich habe Ihnen leider Ihr Angebot nicht wie gewuenscht erstellen koennen, da ab16798 und ab23720 aus unserem Katalog heraus genommen wurden.

    Wollen Sie, dass wir uns fuer Sie nach einer Alternative erkundigen?



    vielen Dank für Ihre Antwort und für Ihre Hilfe beim finden eines Alternativeartikels. Ich benötige die Artikel für Western Blot_murine, human.

    Vielen Dank, beste Grüsse

    Read More
    Answer

    Vielen Dank für Ihre Anfrage.

    Ich habe unseren Produktkatalog für sie durchsucht und möchte Ihnen nun ein Angebot machen. Wir haben leider zur Zeit nur einen AGE Antikörper im Angebot und dieser ist unserem Wissen nach noch nicht für WB und nicht in Maus getestet wurden.

    Ich würde Ihnen daher, wenn Sie sich entschließen diesen Antikörper zu erwerben, gerne unseren Abtrial anbieten. Wenn Sie ab23722 in WB mit Humanen- und Mauspropen testen, bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von €410.00 des ab23722 abdecken, und gegen eine erneute Bestellung eines weiteren Produktes von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte den folgenden Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab23722 testen möchten. Ich werde ihnen dann einen Rabattcode zusenden.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in WB mit Humanen- und Mausproben.

    5.) Teilen Sie uns das Ergebnis Ihres Tests innerhalb von 4 Monaten durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes".
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab23722 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer vonab23722 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% AbTrial Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11998

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Als Ersatzantikörper für die Detektion von Glutathione Peroxidase 1 kann ich Ihnen zwei Vorschläge machen. Ich habe zwei Antikörper gefunden, die sowohl für WB als auch Maus und Human getestet wurden und von unserer 6 monatigen Garantie hierfür abgedeckt werden.

    1. Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)

    Bei diesem Antikoerper handelt es sich um einen RabMab und sollten Sie bis zum 16.12.12 bestellen können Sie von unserer RabMab Aktion Gebrauch machen. Bis zum 16.12.12 bekommen Sie bei der Bestellung eines primären Antikörpers einen RabMab kostenfrei mit dazu wenn Sie bei der Bestellung folgenden Code angeben: RABMAB-XBSMF.

    Das heißt, sollten Sie sich für den RabMab als zweiten Antikörper entscheiden, bekommen Sie diesen kostenfrei! Sie können mehr über dieses Angebot auf folgender Seite nachlesen:

    https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Wir bieten außerdem den Anti-Glutathione Peroxidase 1 antibody (ab22604) an. Dieser schneidet in allen Bewertungen gut an (5 Sterne insgesamt) und ist durch zahlreiche Referenzen sowie Abreviews gut charakterisiert.


    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
    https://www.abcam.com/abreviews

    Read More

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