Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-Glutathione Peroxidase 1 antibody [EPR3312]
See all Glutathione Peroxidase 1 primary antibodies -
DescriptionRabbit monoclonal [EPR3312] to Glutathione Peroxidase 1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Common marmoset
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Immunogen
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Positive control
- Human fetal liver, 293T, THP1, and HepG2 cell lysates; Human kidney tissue; 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR3312
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Glutathione Peroxidase 1 peptide (ab156307). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/6000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionProtects the hemoglobin in erythrocytes from oxidative breakdown.
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Sequence similaritiesBelongs to the glutathione peroxidase family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 2876 Human
- Entrez Gene: 14775 Mouse
- Entrez Gene: 24404 Rat
- Omim: 138320 Human
- SwissProt: Q0EF99 Common marmoset
- SwissProt: P07203 Human
- SwissProt: P11352 Mouse
- SwissProt: P04041 Rat
see all -
Alternative names
- AL033363 antibody
- Cellular glutathione peroxidase antibody
- Glutathione peroxidase 1 antibody
see all
Images
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![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-257928-ab108427KOWB.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
Lane 3: THP1 cell lysate (20 µg)
Lane 4: HL60 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108427 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108427 was shown to recognize Glutathione Peroxidase 1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108427 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-9-ab108427FC1.jpg)
Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Flow cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling Glutathione Peroxidase 1 (red) with purified ab108427 at a 1/6000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-256944-ab108427WB2.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + THP-1 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-256943-ab108427WB1.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + Human fetal liver at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunoprecipitation - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-5-ab108427IP1.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)ab108427 (purified) at 1/40 immunoprecipitating glutathione peroxidase 1 in 10 μg THP-1 cell lysate (Lanes 1 and 2, observed at 22 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST -
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/Glutathione-Peroxidase-1-Primary-antibodies-ab108427-6.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : 293T cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
References
This product has been referenced in:
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). Read more (PubMed: 28736722) »
- Sollanek KJ et al. Role of intrinsic aerobic capacity and ventilator-induced diaphragm dysfunction. J Appl Physiol (1985) 118:849-57 (2015). Read more (PubMed: 25571991) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"