Recombinant Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-472352-anti-glutathione-peroxidase-1-antibody-epr3312-western-blot-wildtype-hek293t-gpx1-knockout-hek293t.jpg "Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3312] to Glutathione Peroxidase 1
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutathione Peroxidase 1 antibody [EPR3312]
See all Glutathione Peroxidase 1 primary antibodies -
Description
Rabbit monoclonal [EPR3312] to Glutathione Peroxidase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Common marmoset
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab156307) -
Positive control
- Human fetal liver, 293T, THP1, and HepG2 whole cell lysate (ab7900); Human kidney tissue; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3312 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Glutathione Peroxidase 1 peptide (ab156307).
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Glutathione Peroxidase 1 peptide (ab156307). |
|
IP
1/10 - 1/100. |
Target
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Function
Protects the hemoglobin in erythrocytes from oxidative breakdown. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2876 Human
- Entrez Gene: 14775 Mouse
- Entrez Gene: 24404 Rat
- Omim: 138320 Human
- SwissProt: Q0EF99 Common marmoset
- SwissProt: P07203 Human
- SwissProt: P11352 Mouse
- SwissProt: P04041 Rat
see all -
Alternative names
- AL033363 antibody
- Cellular glutathione peroxidase antibody
- Glutathione peroxidase 1 antibody
see all
Images
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Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : GPX1 knockout HEK-293T cell lysate
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDaFalse colour image of Western blot: Anti-Glutathione Peroxidase 1 antibody [EPR3312] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108427 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line ab266650 (knockout cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-257928-anti-glutathione-peroxidase-1-antibody-epr3312-western-blot.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
Lane 3: THP1 cell lysate (20 µg)
Lane 4: HL60 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108427 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108427 was shown to recognize Glutathione Peroxidase 1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108427 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-256944-anti-glutathione-peroxidase-1-antibody-epr3312-western-blot.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + THP-1 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-256943-anti-glutathione-peroxidase-1-antibody-epr3312-western-blot.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + Human fetal liver at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunoprecipitation - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-256850-anti-glutathione-peroxidase-1-antibody-epr3312-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)ab108427 (purified) at 1/40 immunoprecipitating glutathione peroxidase 1 in 10 μg THP-1 cell lysate (Lanes 1 and 2, observed at 22 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-121159-anti-glutathione-peroxidase-1-antibody-epr3312-western-blot.jpg)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : 293T cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
![Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)](https://www.abcam.com/ps/products/108/ab108427/Images/ab108427-10-benefits-of-recombinant-antibodies.png)
Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
Protocols
Datasheets and documents
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Datasheet download
References (34)
ab108427 has been referenced in 34 publications.
- Zhang XH et al. Zenglv Fumai Granule protects cardiomyocytes against hypoxia/reoxygenation-induced apoptosis via inhibiting TRIM28 expression. Mol Med Rep 23:N/A (2021). PubMed: 33398366
- Reutzel M et al. Effects of different standard and special diets on cognition and brain mitochondrial function in mice. Nutr Neurosci N/A:1-13 (2021). PubMed: 33814001
- Sun Q et al. PER1 interaction with GPX1 regulates metabolic homeostasis under oxidative stress. Redox Biol 37:101694 (2020). PubMed: 32896721
- Xu M et al. Proteomic Reveals Reasons for Acquired Drug Resistance in Lung Cancer Derived Brain Metastasis Based on a Newly Established Multi-Organ Microfluidic Chip Model. Front Bioeng Biotechnol 8:612091 (2020). PubMed: 33415100
- Koeberle SC et al. Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-?B-driven inflammation through redox-active mechanisms. Redox Biol 28:101388 (2020). PubMed: 31765890
