Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 2/GPX2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glutathione Peroxidase 2/GPX2 antibody
See all Glutathione Peroxidase 2/GPX2 primary antibodies
DescriptionRabbit polyclonal to Glutathione Peroxidase 2/GPX2
Reacts specifically with human 22 kDa Glutathione Peroxidase 2/GPX2 protein
Tested Applications & Species
Application Species IHC-PHuman
Synthetic peptide corresponding to Human Glutathione Peroxidase 2/GPX2.
This product was previously labelled as Glutathione Peroxidase 2
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: Whole serum
Concentration information loading...
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
FunctionCould play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.
Tissue specificityMostly in liver and gastrointestinal tract, not found in heart or kidney.
Sequence similaritiesBelongs to the glutathione peroxidase family.
Cellular localizationCytoplasm. Mainly cytoplasmic.
- Information by UniProt
- Gastrointestinal glutathione peroxidase antibody
- GI GPx antibody
- Glutathione peroxidase 2 (gastrointestinal) antibody
Ab64322 staining human normal bladder tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab64322 has been referenced in 2 publications.
- Cecchini MJ et al. CDX2 and Muc2 immunohistochemistry as prognostic markers in stage II colon cancer. Hum Pathol 90:70-79 (2019). PubMed: 31121192
- Liu T et al. GPX2 overexpression indicates poor prognosis in patients with hepatocellular carcinoma. Tumour Biol 39:1010428317700410 (2017). PubMed: 28635398