Recombinant Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (ab140130)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8175(2)] to Glutathione Peroxidase 2/GPX2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)]
See all Glutathione Peroxidase 2/GPX2 primary antibodies -
Description
Rabbit monoclonal [EPR8175(2)] to Glutathione Peroxidase 2/GPX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Glutathione Peroxidase 2/GPX2 aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- Human colon cancer and stomach lysates; Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8175(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. -
Tissue specificity
Mostly in liver and gastrointestinal tract, not found in heart or kidney. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Cytoplasm. Mainly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 2877 Human
- Omim: 138319 Human
- SwissProt: P18283 Human
- Unigene: 2704 Human
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Alternative names
- Gastrointestinal glutathione peroxidase antibody
- GI GPx antibody
- Glutathione peroxidase 2 (gastrointestinal) antibody
see all
Images
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All lanes : Anti-Glutathione Peroxidase 2/GPX2 antibody [EPR8175(2)] (ab140130) at 1/1000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Glutathione Peroxidase 2/GPX2 with ab140130 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab140130 has been referenced in 5 publications.
- Song H et al. Methyltransferase like 7B is a potential therapeutic target for reversing EGFR-TKIs resistance in lung adenocarcinoma. Mol Cancer 21:43 (2022). PubMed: 35144642
- Yang M et al. GPX2 predicts recurrence-free survival and triggers the Wnt/β-catenin/EMT pathway in prostate cancer. PeerJ 10:e14263 (2022). PubMed: 36312753
- Wang Y et al. GPX2 suppression of H2O2 stress regulates cervical cancer metastasis and apoptosis via activation of the ß-catenin-WNT pathway. Onco Targets Ther 12:6639-6651 (2019). PubMed: 31695405
- Touat-Hamici Z et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . PubMed: 29660373
- Geraghty P et al. Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure. Mediators Inflamm 2016:9461289 (2016). PubMed: 28070146