Overview

  • Product name

    Anti-Glutathione Peroxidase 3/GPx-3 antibody
    See all Glutathione Peroxidase 3/GPx-3 primary antibodies
  • Description

    Goat polyclonal to Glutathione Peroxidase 3/GPx-3
  • Host species

    Goat
  • Specificity

    ab59524 may crossreact with epididymis-specific Glutathione peroxidase 5 (GPX5) and olfactory epithelium-speciific Glutathione peroxidase 6 (GPX6).

  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Pig
    Predicted to work with: Cow, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human Glutathione Peroxidase 3/GPx-3 aa 102-114 (internal sequence).
    Sequence:

    C-NQFGKQEPGENSE

  • Positive control

    • Recombinant Human Glutathione Peroxidase 3/GPx-3 protein (ab123158) can be used as a positive control in WB. Mouse Heart, Kidney and Liver lysates.
  • General notes

    Previously labelled as Glutathione Peroxidase 3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab59524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-Fr Use a concentration of 20 µg/ml.

Target

  • Function

    Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
  • Tissue specificity

    Secreted in plasma.
  • Sequence similarities

    Belongs to the glutathione peroxidase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Extracellular glutathione peroxidase antibody
    • Glutathione peroxidase 3 (plasma) antibody
    • Glutathione peroxidase 3 antibody
    • GPX 3 antibody
    • GPx P antibody
    • GPx-3 antibody
    • GPx-P antibody
    • GPX3 antibody
    • GPX3_HUMAN antibody
    • GPXP antibody
    • GSHPx 3 antibody
    • GSHPx P antibody
    • GSHPx-3 antibody
    • GSHPx-P antibody
    • Plasma glutathione peroxidase 3 antibody
    • Plasma glutathione peroxidase antibody
    see all

Images

  • ab59524 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining showing vesicular staining of the cytoplasm in PCT epithelial cells.

  • Anti-Glutathione Peroxidase 3/GPx-3 antibody (ab59524) at 1 µg/ml + Mouse Heart lysate in RIPA buffer at 35 µg

    Predicted band size: 25 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Detected by chemiluminescence

References

This product has been referenced in:

  • Civantos E  et al. Sitagliptin ameliorates oxidative stress in experimental diabetic nephropathy by diminishing the miR-200a/Keap-1/Nrf2 antioxidant pathway. Diabetes Metab Syndr Obes 10:207-222 (2017). WB ; Rat . Read more (PubMed: 28652790) »
  • Lubieniecka JM  et al. Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats. PLoS One 6:e19247 (2011). WB ; Rat . Read more (PubMed: 21559420) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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