Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.com/ps/products/16/ab16739/Images/ab16739-297384-anti-glutathione-peroxidase-4-antibody-1h11-immunoprecipitation.jpg "Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)")
Key features and details
- Mouse monoclonal [1H11] to Glutathione Peroxidase 4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Glutathione Peroxidase 4 antibody [1H11]
See all Glutathione Peroxidase 4 primary antibodies -
Description
Mouse monoclonal [1H11] to Glutathione Peroxidase 4 -
Host species
Mouse -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein purified from E.coli (Human).
-
Positive control
- HL60 cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
1H11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use a concentration of 1 - 2 µg/ml.
|
| Notes |
|---|
|
IP
Use a concentration of 1 - 2 µg/ml. |
Target
-
Function
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
Tissue specificity
Present primarily in testis. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Mitochondrion. Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2879 Human
- Omim: 138322 Human
- SwissProt: P36969 Human
- Unigene: 433951 Human
-
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
Images
-
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175
All lanes :
Lane 1 : 239T Cell Lysate
Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
Lane 4 : Precipitated from PBS (using 5 ug ab16739)
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution -
![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.com/ps/products/16/ab16739/Images/ab16739-25589-anti-glutathione-peroxidase-4-antibody-1h11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Western blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.
Datasheets and documents
-
Datasheet download
References (2)
ab16739 has been referenced in 2 publications.
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
- Yang L et al. Broad Spectrum Deubiquitinase Inhibition Induces Both Apoptosis and Ferroptosis in Cancer Cells. Front Oncol 10:949 (2020). PubMed: 32596160
