Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Glutathione Peroxidase 4 antibody [1H11]
See all Glutathione Peroxidase 4 primary antibodies -
Description
Mouse monoclonal [1H11] to Glutathione Peroxidase 4 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein purified from E.coli (Human).
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Positive control
- HL60 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
1H11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| IP | Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
Tissue specificity
Present primarily in testis. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Mitochondrion. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2879 Human
- Entrez Gene: 625249 Mouse
- Entrez Gene: 29328 Rat
- Omim: 138322 Human
- SwissProt: P36969 Human
- SwissProt: O70325 Mouse
- SwissProt: P36970 Rat
- Unigene: 433951 Human
see all -
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
Images
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![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.com/ps/products/16/ab16739/Images/ab16739-297384-anti-glutathione-peroxidase-4-antibody-1h11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175
All lanes :
Lane 1 : 239T Cell Lysate
Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
Lane 4 : Precipitated from PBS (using 5 ug ab16739)
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution -
![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.com/ps/products/16/ab16739/Images/ab16739-25589-anti-glutathione-peroxidase-4-antibody-1h11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Western blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.
Protocols
Datasheets and documents
References
ab16739 has not yet been referenced specifically in any publications.
