Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

Product name

Anti-Glutathione Peroxidase 4 antibody [1H11]
See all Glutathione Peroxidase 4 primary antibodies
Description

Mouse monoclonal [1H11] to Glutathione Peroxidase 4
Host species

Mouse
Tested applications

Suitable for: ELISA, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant full length protein purified from E.coli (Human).
Positive control
- HL60 cell lysate

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

1H11
Isotype

IgG1
Light chain type

kappa
Research areas

Our Abpromise guarantee covers the use of ab16739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ELISA		Use at an assay dependent dilution.
IP		Use a concentration of 1 - 2 µg/ml.

Function

Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
Tissue specificity

Present primarily in testis.
Sequence similarities

Belongs to the glutathione peroxidase family.
Cellular localization

Mitochondrion. Cytoplasm.
Target information above from: UniProt accession P36969 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2879 Human
- Entrez Gene: 625249 Mouse
- Entrez Gene: 29328 Rat
- Omim: 138322 Human
- SwissProt: P36969 Human
- SwissProt: O70325 Mouse
- SwissProt: P36970 Rat
- Unigene: 433951 Human
- Unigene: 359573 Mouse
- Unigene: 3647 Rat
see all
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
- GPX4 antibody
- GPX4_HUMAN antibody
- GSHPx-4 antibody
- MCSP antibody
- mitochondrial antibody
- PHGPx antibody
- Phospholipid hydroperoxidase antibody
- Phospholipid hydroperoxide glutathione peroxidase antibody
- Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
- snGPx antibody
- snPHGPx antibody
- Sperm nucleus glutathione peroxidase antibody
see all

Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

All lanes :

Lane 1 : 239T Cell Lysate
Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
Lane 4 : Precipitated from PBS (using 5 ug ab16739)

Secondary
All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Western blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.

ab16739 has not yet been referenced specifically in any publications.

Publishing research using ab16739? Please let us know so that we can cite the reference in this datasheet.

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