Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

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Overview

  • Product name
    Anti-Glutathione Peroxidase 4 antibody [1H11]
    See all Glutathione Peroxidase 4 primary antibodies
  • Description
    Mouse monoclonal [1H11] to Glutathione Peroxidase 4
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein purified from E.coli (Human).

  • Positive control
    • HL60 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab16739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use a concentration of 1 - 2 µg/ml.

Target

  • Function
    Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
  • Tissue specificity
    Present primarily in testis.
  • Sequence similarities
    Belongs to the glutathione peroxidase family.
  • Cellular localization
    Mitochondrion. Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Glutathione peroxidase 4 antibody
    • GPX 4 antibody
    • GPX-4 antibody
    • GPX4 antibody
    • GPX4_HUMAN antibody
    • GSHPx-4 antibody
    • MCSP antibody
    • mitochondrial antibody
    • PHGPx antibody
    • Phospholipid hydroperoxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
    • snGPx antibody
    • snPHGPx antibody
    • Sperm nucleus glutathione peroxidase antibody
    see all

Images

  • Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
    Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175



    All lanes :

    Lane 1 : 239T Cell Lysate
    Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
    Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
    Lane 4 : Precipitated from PBS (using 5 ug ab16739)

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution
  • Western blot - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
    Western blot - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

    Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Westrn blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.

    Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Westrn blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.

References

ab16739 has not yet been referenced specifically in any publications.

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