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    glutathione-peroxidase-4-antibody-1h11-ab16739.pdf

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Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

  • Datasheet
Reviews (1) Submit a question References (2)

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Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
  • Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

Key features and details

  • Mouse monoclonal [1H11] to Glutathione Peroxidase 4
  • Suitable for: IP
  • Reacts with: Human
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Glutathione Peroxidase 4 antibody [1H11]
    See all Glutathione Peroxidase 4 primary antibodies
  • Description

    Mouse monoclonal [1H11] to Glutathione Peroxidase 4
  • Host species

    Mouse
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein purified from E.coli (Human).

  • Positive control

    • HL60 cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    1H11
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use a concentration of 1 - 2 µg/ml.
Notes
IP
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
  • Tissue specificity

    Present primarily in testis.
  • Sequence similarities

    Belongs to the glutathione peroxidase family.
  • Cellular localization

    Mitochondrion. Cytoplasm.
  • Target information above from: UniProt accession P36969 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2879 Human
    • Omim: 138322 Human
    • SwissProt: P36969 Human
    • Unigene: 433951 Human
    • Alternative names

      • Glutathione peroxidase 4 antibody
      • GPX 4 antibody
      • GPX-4 antibody
      • GPX4 antibody
      • GPX4_HUMAN antibody
      • GSHPx-4 antibody
      • MCSP antibody
      • mitochondrial antibody
      • PHGPx antibody
      • Phospholipid hydroperoxidase antibody
      • Phospholipid hydroperoxide glutathione peroxidase antibody
      • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
      • snGPx antibody
      • snPHGPx antibody
      • Sperm nucleus glutathione peroxidase antibody
      see all

    Images

    • Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
      Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

      Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175



      All lanes :

      Lane 1 : 239T Cell Lysate
      Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
      Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
      Lane 4 : Precipitated from PBS (using 5 ug ab16739)

      Secondary
      All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution
    • Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
      Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)

      Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Western blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.

    Protocols

    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab16739? Please let us know so that we can cite the reference in this datasheet.

    ab16739 has been referenced in 2 publications.

    • Guo S  et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
    • Yang L  et al. Broad Spectrum Deubiquitinase Inhibition Induces Both Apoptosis and Ferroptosis in Cancer Cells. Front Oncol 10:949 (2020). PubMed: 32596160

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Glutathione Peroxidase 4 antibody [1H11]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Thyroid cancer cell line K1)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS-PAGE)
    Loading amount
    20 µg
    Specification
    Thyroid cancer cell line K1
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    sekhar konjeti

    Verified customer

    Submitted Jun 10 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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