Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Glutathione Peroxidase 4 antibody [1H11]
See all Glutathione Peroxidase 4 primary antibodies -
DescriptionMouse monoclonal [1H11] to Glutathione Peroxidase 4
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant full length protein purified from E.coli (Human).
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Positive control
- HL60 cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
PurityProtein G purified
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Purification notesPurified from TCS
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ClonalityMonoclonal
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Clone number1H11
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab16739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| IP | Use a concentration of 1 - 2 µg/ml. |
Target
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FunctionProtects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
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Tissue specificityPresent primarily in testis.
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Sequence similaritiesBelongs to the glutathione peroxidase family.
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Cellular localizationMitochondrion. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 2879 Human
- Entrez Gene: 625249 Mouse
- Entrez Gene: 29328 Rat
- Omim: 138322 Human
- SwissProt: P36969 Human
- SwissProt: O70325 Mouse
- SwissProt: P36970 Rat
- Unigene: 433951 Human
see all -
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
Images
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![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.com/ps/products/16/ab16739/Images/ab16739-297384-ab16739-1H11-IP.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175
All lanes :
Lane 1 : 239T Cell Lysate
Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
Lane 4 : Precipitated from PBS (using 5 ug ab16739)
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution -
![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.com/ps/products/16/ab16739/Images/ab16739_1.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Western blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.
Protocols
References
ab16739 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"