Product nameAnti-Glutathione Peroxidase 4 antibody
See all Glutathione Peroxidase 4 primary antibodies
DescriptionRabbit polyclonal to Glutathione Peroxidase 4
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
- ab40993 gave a positive signal in the following human tissue lysates: Brain, Testis, Liver, Heart, Skeletal Muscle, Kidney, Colon, Small Inestine (data not shown).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab40993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
FunctionProtects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
Tissue specificityPresent primarily in testis.
Sequence similaritiesBelongs to the glutathione peroxidase family.
Cellular localizationMitochondrion. Cytoplasm.
- Information by UniProt
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
All lanes : Anti-Glutathione Peroxidase 4 antibody (ab40993) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lane 7 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
ICC/IF image of ab40993 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40993, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
This product has been referenced in:
- Hauser DN et al. Dopamine quinone modifies and decreases the abundance of the mitochondrial selenoprotein glutathione peroxidase 4. Free Radic Biol Med 65:419-27 (2013). Read more (PubMed: 23816523) »
- Li W et al. Repression of endometrial tumor growth by targeting SREBP1 and lipogenesis. Cell Cycle 11:2348-58 (2012). Read more (PubMed: 22672904) »