Name: Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
Brand: RabMAb
SKU: ab125066
Rating: 5 (3 reviews)

Product name

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
See all Glutathione Peroxidase 4 primary antibodies
Description

Rabbit monoclonal [EPNCIR144] to Glutathione Peroxidase 4
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Mouse Glutathione Peroxidase 4. The exact sequence is proprietary.
Database link: O70325
Positive control
- WB: Mouse testis, rat testis, human testis, human fetal liver, and human seminoma tissue lysates, LnCaP, Jurkat and HepG2 cell lysates. ICC/IF: HEK293 cells. IHC-P: Human kidney and stomach tissues. Flow Cyt: HeLa cells.
General notes

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor^® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPNCIR144
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
- Jurkat cytoplasmic extract lysate (ab14842)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Our Abpromise guarantee covers the use of ab125066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/400.
WB		1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IHC-P		1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF		1/200.

Function

Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
Tissue specificity

Present primarily in testis.
Sequence similarities

Belongs to the glutathione peroxidase family.
Cellular localization

Mitochondrion. Cytoplasm.
Target information above from: UniProt accession P36969 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2879 Human
- Entrez Gene: 625249 Mouse
- Entrez Gene: 29328 Rat
- Omim: 138322 Human
- SwissProt: P36969 Human
- SwissProt: O70325 Mouse
- SwissProt: P36970 Rat
- Unigene: 433951 Human
- Unigene: 359573 Mouse
- Unigene: 3647 Rat
see all
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
- GPX4 antibody
- GPX4_HUMAN antibody
- GSHPx-4 antibody
- MCSP antibody
- mitochondrial antibody
- PHGPx antibody
- Phospholipid hydroperoxidase antibody
- Phospholipid hydroperoxide glutathione peroxidase antibody
- Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
- snGPx antibody
- snPHGPx antibody
- Sperm nucleus glutathione peroxidase antibody
see all

Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/20000 dilution (purified)

Lane 1 : mouse testis lysate
Lane 2 : rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 22 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

Unpurified ab125066, at a 1/100 dilution, staining Glutathione Peroxidase 4 in paraffin-embedded Human kidney tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

Immunofluorescence staining of HEK293 cells with purified ab125066 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab125066 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Flow Cytometry - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Glutathione Peroxidase 4 (red) with ab125066 at a 1/400 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/20000 dilution (purified)

Lane 1 : human fetal liver lysate
Lane 2 : Jurkat lysate

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

Immunohistochemical staining of paraffin embedded human stomach with purified ab125066 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)This image is courtesy of an Abreview submitted by Kirk McManus

Unpurified ab125066 staining Glutathione Peroxidase 4 in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081 an Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Nuclear staining was carried out with DAPI.
See Abreview
Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution (unpurified)

Lane 1 : Human testis lysate
Lane 2 : Human seminoma lysate
Lane 3 : LnCaP cell lysate
Lane 4 : Human fetal liver lysate
Lane 5 : Jurkat cell lysate
Lane 6 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 22 kDa

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Simanjuntak Y et al. Ebselen alleviates testicular pathology in mice with Zika virus infection and prevents its sexual transmission. PLoS Pathog 14:e1006854 (2018). Read more (PubMed: 29447264) »
Touat-Hamici Z et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . Read more (PubMed: 29660373) »

See all 26 Publications for this product

Publishing research using ab125066? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Mouse Cell lysate - other (Hepa 1-6 (hepatocellular carcinoma cell line))

Gel Running Conditions

Reduced Denaturing (15% gel)

Loading amount

10 µg

Specification

Hepa 1-6 (hepatocellular carcinoma cell line)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Takujiro Homma

Verified customer

Submitted Dec 18 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (head and neck cancer)

Gel Running Conditions

Non-reduced Non-Denaturing (Native) (10% gel)

Loading amount

20 µg

Treatment

cystine deficient medium, glutamine high medium 48h

Specification

head and neck cancer

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 02 2015

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Permeabilization

Fixative

Methanol

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 05 2014

Question

Mouse, Frozen section. Testing discount

Abcam community

Verified customer

Asked on Dec 17 2012

Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde der ab125066 Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] bisher noch nicht in IHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab125066 abdecken, und gegen eine neue Bestellung von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab125066 in IHC-Fr testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in IHC-Fr.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv.Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Abcam Scientific Support

Answered on Dec 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Location selector

Your location is currently set to:

Custom solutions & partnerships

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Western blot abreview for Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

Western blot abreview for Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]