Recombinant
RabMAb

Recombinant Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (HRP) (ab206266)

Overview

  • Product name

    Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (HRP)
    See all Glutathione Peroxidase 4 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR144] to Glutathione Peroxidase 4 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse Glutathione Peroxidase 4. The exact sequence is proprietary.
    Database link: O70325

  • Positive control

    • WB: Human Testis and Fetal Liver tissue lysates; Jurkat and LNCaP whole cell lysates.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab206266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 17 kDa).

Target

  • Function

    Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
  • Tissue specificity

    Present primarily in testis.
  • Sequence similarities

    Belongs to the glutathione peroxidase family.
  • Cellular localization

    Mitochondrion. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glutathione peroxidase 4 antibody
    • GPX 4 antibody
    • GPX-4 antibody
    • GPX4 antibody
    • GPX4_HUMAN antibody
    • GSHPx-4 antibody
    • MCSP antibody
    • mitochondrial antibody
    • PHGPx antibody
    • Phospholipid hydroperoxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
    • snGPx antibody
    • snPHGPx antibody
    • Sperm nucleus glutathione peroxidase antibody
    see all

Images

  • All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (HRP) (ab206266) at 1/5000 dilution

    Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206266 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab206266 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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