Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR144] to Glutathione Peroxidase 4 (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-Glutathione Peroxidase 4 antibody [EPNCIR144] (HRP)
See all Glutathione Peroxidase 4 primary antibodies
DescriptionRabbit monoclonal [EPNCIR144] to Glutathione Peroxidase 4 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Mouse Glutathione Peroxidase 4. The exact sequence is proprietary.
Database link: O70325
- WB: Human Testis and Fetal Liver tissue lysates; Jurkat and LNCaP whole cell lysates.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab206266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 17 kDa).|
FunctionProtects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
Tissue specificityPresent primarily in testis.
Sequence similaritiesBelongs to the glutathione peroxidase family.
Cellular localizationMitochondrion. Cytoplasm.
- Information by UniProt
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (HRP) (ab206266) at 1/5000 dilution
Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue
Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206266 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab206266 has not yet been referenced specifically in any publications.