Overview

  • Product name

    Glutathione Peroxidase Activity Assay Kit (Fluorometric)
    See all Glutathione Peroxidase kits
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate
  • Assay type

    Enzyme activity
  • Sensitivity

    < 1.25 mU/ml
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Glutathione Peroxidase Activity Assay Kit (Fluorometric) (ab219926) provides a simple method to measure Glutathione Peroxidase activity in cell lysates. This assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG) catalyzed by Glutathione Peroxidase (GPx). The generated GSSG is recycled to its reduced state GSH by glutathione reductase (GR) and NADPH. The reaction product, NADP+, can be specifically monitored using our newly developed proprietary NADP sensor, which reacts with NADP only to generate a fluorescent product. The signal, which is proportional to the GPx activity present in the sample, can be measured with a fluorescence microplate reader at Ex/Em = 420/480 nm.


    This assay can detect activity from as low as 1.25 mU/mL Glutathione Peroxidase in solution. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.

  • Notes

    Glutathione peroxidase (GPx) is an enzyme family with peroxidase activity to protect the organism from oxidative damage. GPx plays an important role in reducing organic hydroperoxides such as lipid hydroperoxides to their corresponding alcohols, or reducing free hydrogen peroxide to water. It therefore guards against oxidative damage to the cell membranes and other oxidant-sensitive sites in the cell. It has been noticed that altered GPx levels correlate with lesions caused by many comment and complex diseases. GPx level is measured in biological samples as a potential indicator for the potential treatment of cancer, diabetes, neurodegenerative and cardiovascular diseases.

  • Platform

    Microplate reader

Properties

Images

  • Typical Glutathione Peroxidase dose response curve. Fluorescence was measured on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices). As low as 1.25 mU/mL GPx can be detected with 30-60 minutes incubation.

Protocols

References

ab219926 has not yet been referenced specifically in any publications.

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