Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 1.25 mU/ml
Product nameGlutathione Peroxidase Activity Assay Kit (Fluorometric)
See all Glutathione Peroxidase kits
Sample typeCell Lysate
Assay typeEnzyme activity
Sensitivity< 1.25 mU/ml
Species reactivityReacts with: Mammals, Other species
Glutathione Peroxidase Activity Assay Kit (Fluorometric) (ab219926) provides a simple method to measure Glutathione Peroxidase activity in cell lysates. This assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG) catalyzed by Glutathione Peroxidase (GPx). The generated GSSG is recycled to its reduced state GSH by glutathione reductase (GR) and NADPH. The reaction product, NADP+, can be specifically monitored using our newly developed proprietary NADP sensor, which reacts with NADP only to generate a fluorescent product. The signal, which is proportional to the GPx activity present in the sample, can be measured with a fluorescence microplate reader at Ex/Em = 420/480 nm.
This assay can detect activity from as low as 1.25 mU/mL Glutathione Peroxidase in solution. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.
Glutathione peroxidase (GPx) is an enzyme family with peroxidase activity to protect the organism from oxidative damage. GPx plays an important role in reducing organic hydroperoxides such as lipid hydroperoxides to their corresponding alcohols, or reducing free hydrogen peroxide to water. It therefore guards against oxidative damage to the cell membranes and other oxidant-sensitive sites in the cell. It has been noticed that altered GPx levels correlate with lesions caused by many comment and complex diseases. GPx level is measured in biological samples as a potential indicator for the potential treatment of cancer, diabetes, neurodegenerative and cardiovascular diseases.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 10ml Enhancer Solution 1 x 3.5ml Enzyme Mix 2 vials Glutathione Peroxidase Standard (0.5 U) 1 vial GSH (3 mg) 1 vial NADP Assay Solution 1 x 5ml NADP Probe 1 x 5ml Substrate 1 x 11µl
- Kits/ Lysates/ Other
- Cell Metabolism Kits
- Coenzyme and cofactor assay kits
- Glutathione assay kits
RelevanceGlutathione Peroxidase (GPx, EC 184.108.40.206) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.
ab219926 has been referenced in 2 publications.
- Mett J & Müller U The medium-chain fatty acid decanoic acid reduces oxidative stress levels in neuroblastoma cells. Sci Rep 11:6135 (2021). PubMed: 33731759
- Saxena S et al. Connective tissue fibroblasts from highly regenerative mammals are refractory to ROS-induced cellular senescence. Nat Commun 10:4400 (2019). PubMed: 31562333