Overview

  • Product name

    Anti-Glutathione Reductase antibody
    See all Glutathione Reductase primary antibodies
  • Description

    Rabbit polyclonal to Glutathione Reductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human Glutathione Reductase aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab95921)

  • Positive control

    • This antibody gave a positive signal in HeLa and Jurkat whole cell lysates and in the following human tissue lysates: Brain; Liver; Testis; Lung; Heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab87330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    Maintains high levels of reduced glutathione in the cytosol.
  • Sequence similarities

    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Domain

    Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface.
  • Cellular localization

    Cytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • GLR1 antibody
    • GLUR antibody
    • Glutathione reductase antibody
    • Glutathione reductase mitochondrial antibody
    • Glutathione reductase, mitochondrial antibody
    • GR antibody
    • Gr1 antibody
    • GRase antibody
    • GRD 1 antibody
    • GRD1 antibody
    • GSHR_HUMAN antibody
    • GSR antibody
    • MGC78522 antibody
    see all

Images

  • All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : GSR knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab87330 observed at 56 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab87330 was shown to recognize Glutathione Reductase in wild-type HAP1 cells as signal was lost at the expected MW in GSR knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSR knockout samples were subjected to SDS-PAGE. Ab87330 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)
    Lane 6 : Lung (Human) Tissue Lysate
    Lane 7 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 37 kDa, 50 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds

References

ab87330 has not yet been referenced specifically in any publications.

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