Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Glutathione Reductase antibody [EPR7237] (ab128933)

Overview

  • Product name

    Anti-Glutathione Reductase antibody [EPR7237]
    See all Glutathione Reductase primary antibodies
  • Description

    Rabbit monoclonal [EPR7237] to Glutathione Reductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Glutathione Reductase (P00390)

  • Positive control

    • Caco-2, A549 or HeLa lysate, Caco-2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Maintains high levels of reduced glutathione in the cytosol.
    • Sequence similarities

      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    • Domain

      Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface.
    • Cellular localization

      Cytoplasm and Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • GLR1 antibody
      • GLUR antibody
      • Glutathione reductase antibody
      • Glutathione reductase mitochondrial antibody
      • Glutathione reductase, mitochondrial antibody
      • GR antibody
      • Gr1 antibody
      • GRase antibody
      • GRD 1 antibody
      • GRD1 antibody
      • GSHR_HUMAN antibody
      • GSR antibody
      • MGC78522 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab128933 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab128933 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. Ab128933 and ab8245 (loading control to GAPDH) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Glutathione Reductase antibody [EPR7237] (ab128933) at 1/10000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat

      anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    • ab128933, at a 1/100 dilution, staining Human Glutathione Reductase in HeLa cells, by Immunofluorescence.

    References

    This product has been referenced in:

    • Dey S  et al. Compartment-specific Control of Reactive Oxygen Species Scavenging by Antioxidant Pathway Enzymes. J Biol Chem 291:11185-97 (2016). Read more (PubMed: 27048652) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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