Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)

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Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
  • Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
  • Immunocytochemistry - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
  • Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
  • Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7237] to Glutathione Reductase - BSA and Azide free
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free
    See all Glutathione Reductase primary antibodies

  • Description

    Rabbit monoclonal [EPR7237] to Glutathione Reductase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Glutathione Reductase aa 1-100. The exact sequence is proprietary.
    Database link: P00390

  • Positive control

    • Flow Cyt: HeLa cells

  • General notes

    ab244234 is the carrier-free version of ab128933 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab244234 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab244234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Maintains high levels of reduced glutathione in the cytosol.

    • Sequence similarities

      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

    • Domain

      Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface.

    • Cellular localization

      Cytoplasm and Mitochondrion.
    • Information by UniProt

    • Database links

      • Alternative names

        • GLR1 antibody
        • GLUR antibody
        • Glutathione reductase antibody
        • Glutathione reductase mitochondrial antibody
        • Glutathione reductase, mitochondrial antibody
        • GR antibody
        • Gr1 antibody
        • GRase antibody
        • GRD 1 antibody
        • GRD1 antibody
        • GSHR_HUMAN antibody
        • GSR antibody
        • MGC78522 antibody
        see all

      Images

      • Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)

        This data was developed using ab128933, the same antibody clone in a different buffer formulation.

        Lane 1: Wild-type HAP1 cell lysate (20 µg)

        Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)

        Lane 3: HeLa cell lysate (20 µg)

        Lane 4: Jurkat cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab128933 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab128933 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. ab128933 and ab8245 (loading control to GAPDH) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

      • Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        This data was developed using ab128933, the same antibody clone in a different buffer formulation.
        Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Glutathione Reductase with purified ab128933 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
      • Immunocytochemistry - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        Immunocytochemistry - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        This data was developed using ab128933, the same antibody clone in a different buffer formulation.ab128933, at a 1/100 dilution, staining Human Glutathione Reductase in HeLa cells, by Immunofluorescence.
      • Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        All lanes : Anti-Glutathione Reductase antibody [EPR7237] (ab128933) at 1/10000 dilution

        Lane 1 : Caco-2 cell lysate
        Lane 2 : A549 cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 56 kDa
        Observed band size: 50 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab128933, the same antibody clone in a different buffer formulation.

      • Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)
        Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (ab244234)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab244234? Please let us know so that we can cite the reference in this datasheet.

      ab244234 has not yet been referenced specifically in any publications.

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