Overview

  • Product name
    Glutathione Reductase (GR) Assay Kit
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay type
    Enzyme activity
  • Sensitivity
    > 0.1 mU/ml
  • Range
    0.1 mU/ml - 40 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

Properties

Images

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Glutathione Reductase assay time line using ab83461.
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    TNB Standard Curve using ab83461.

Protocols

References

This product has been referenced in:
  • Szabó K  et al. Fenugreek (Trigonella Foenum-Graecum) Seed Flour and Diosgenin Preserve Endothelium-Dependent Arterial Relaxation in a Rat Model of Early-Stage Metabolic Syndrome. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29534453) »
  • Cao Z  et al. Isochlorogenic acid C prevents enterovirus 71 infection via modulating redox homeostasis of glutathione. Sci Rep 7:16278 (2017). Read more (PubMed: 29176678) »

See all 3 Publications for this product

Publishing research using ab83461? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

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Inquiry: Hello, I am considering ordering Glutathione Reductase (GR) Assay Kit (ab83461) to study this enzyme activity. I have frozen rabbit placenta. Can you advise me of the details / protocol of preparing this tissue for this analysis (ie tissue ext...

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Thank you for contacting us.

The procedure is given in protocol booklet.

https://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on...

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Thank you for your reply.

I wish to detect the presence of antioxidant enzymes in the tissue (glutathione peroxidase and reductase, SOD and catalase most important) but also the quantification or activity of each enzyme is important. Essentia...

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Thank you for contacting us.

Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use ar...

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I am inquiring about your total antioxidant capacity assay kit or similar.

I have frozen placental tissue from rabbit experiments and I am wishing to examine markers of oxidative stress.
The most important markers for me are glutathione pe...

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Thank you for contacting us.

Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

I will be looking forward to hearing from you soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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