Glutathione Reductase (GR) Assay Kit (ab83461)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mU/ml
Overview
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Product name
Glutathione Reductase (GR) Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Sensitivity
> 0.1 mU/ml -
Range
0.1 mU/ml - 40 mU/ml -
Assay time
0h 40m -
Product overview
Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
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Notes
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
Related products
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 200 tests Catalase (lyophilized) Clear 1 x 1ng DTNB (lyophilized) Red 1 vial GR Assay Buffer NM 1 x 100ml GR Positive Control (10 mU; lyophilized) Green 1 vial GSSG (lyophilized) Yellow 1 vial Hydrogen peroxide Orange 1 x 1ml NADPH-GNERAT™ (lyophilized) Blue 2 vials TNB Standard (2.5 µmol) Brown 1 x 1ng -
Research areas
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Relevance
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. -
Cellular localization
Cytoplasmic and Mitochondrial -
Alternative names
- EC 1.8.1.7
- Glutathione reductase mitochondrial
- GR
see all
Associated products
Images
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Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)Cao, Zeyu et al., Scientific reports?vol. 7,1 16278., Fig 5, doi:10.1038/s41598-017-16446-7ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione Reductase assay time line using ab83461. -
Functional Studies - Glutathione Reductase Assay Kit (ab83461)TNB Standard Curve using ab83461.
References (12)
ab83461 has been referenced in 12 publications.
- Bauckneht M et al. FDG uptake tracks the oxidative damage in diabetic skeletal muscle: An experimental study. Mol Metab 31:98-108 (2020). PubMed: 31918925
- Lechowicz K et al. Two Festuca Species-F. arundinacea and F. glaucescens-Differ in the Molecular Response to Drought, While Their Physiological Response Is Similar. Int J Mol Sci 21:N/A (2020). PubMed: 32365894
- Al-Otaibi NAS et al. Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives. Gigascience 8:N/A (2019). PubMed: 30535373
- Al-Otaibi NAS et al. Cryopreservation of Red Blood Cells. Methods Mol Biol 1916:233-238 (2019). PubMed: 30535700
- Al-Otaibi NAS et al. Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryopreservation. Methods Mol Biol 1916:239-247 (2019). PubMed: 30535701
