Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mU/ml
Product nameGlutathione Reductase (GR) Assay Kit
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Assay typeEnzyme activity
Sensitivity> 0.1 mU/ml
Range0.1 mU/ml - 40 mU/ml
Assay time0h 40m
Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Glutathione Reductase (GR, EC 184.108.40.206) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 200 tests Catalase (lyophilized) Clear 1 x 1ng DTNB (lyophilized) Red 1 vial GR Assay Buffer NM 1 x 100ml GR Positive Control (10 mU; lyophilized) Green 1 vial GSSG (lyophilized) Yellow 1 vial Hydrogen peroxide Orange 1 x 1ml NADPH-GNERAT™ (lyophilized) Blue 2 vials TNB Standard (2.5 µmol) Brown 1 x 1ng
RelevanceGlutathione Reductase (GR, EC 220.127.116.11) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
Cellular localizationCytoplasmic and Mitochondrial
- EC 18.104.22.168
- Glutathione reductase mitochondrial
ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).
Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)
Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.
Glutathione Reductase assay time line using ab83461.
TNB Standard Curve using ab83461.
ab83461 has been referenced in 12 publications.
- Bauckneht M et al. FDG uptake tracks the oxidative damage in diabetic skeletal muscle: An experimental study. Mol Metab 31:98-108 (2020). PubMed: 31918925
- Lechowicz K et al. Two Festuca Species-F. arundinacea and F. glaucescens-Differ in the Molecular Response to Drought, While Their Physiological Response Is Similar. Int J Mol Sci 21:N/A (2020). PubMed: 32365894
- Al-Otaibi NAS et al. Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives. Gigascience 8:N/A (2019). PubMed: 30535373
- Al-Otaibi NAS et al. Cryopreservation of Red Blood Cells. Methods Mol Biol 1916:233-238 (2019). PubMed: 30535700
- Al-Otaibi NAS et al. Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryopreservation. Methods Mol Biol 1916:239-247 (2019). PubMed: 30535701