Glutathione Reductase (GR) Assay Kit (ab83461)

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Overview

  • Product name

    Glutathione Reductase (GR) Assay Kit

  • Detection method

    Colorimetric

  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate

  • Assay type

    Enzyme activity

  • Sensitivity

    > 0.1 mU/ml

  • Range

    0.1 mU/ml - 40 mU/ml

  • Assay time

    0h 40m

  • Product overview

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.

  • Notes

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)Cao, Zeyu et al., Scientific reports?vol. 7,1 16278., Fig 5, doi:10.1038/s41598-017-16446-7
    ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Glutathione Reductase assay time line using ab83461.
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    TNB Standard Curve using ab83461.

Protocols

References

This product has been referenced in:

  • Al-Otaibi NAS  et al. Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives. Gigascience 8:N/A (2019). Read more (PubMed: 30535373) »
  • Al-Otaibi NAS  et al. Cryopreservation of Red Blood Cells. Methods Mol Biol 1916:233-238 (2019). Read more (PubMed: 30535700) »
See all 10 Publications for this product

Publishing research using ab83461? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: Hello, I am considering ordering Glutathione Reductase (GR) Assay Kit (ab83461) to study this enzyme activity. I have frozen rabbit placenta. Can you advise me of the details / protocol of preparing this tissue for this analysis (ie tissue extract), so that I can confirm my lab has the ability to do this. Do you have knowledge whether this has been done before in this type of tissue and do you anticipate any difficulties? Many thanks,

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Answer

Thank you for contacting us.

The procedure is given in protocol booklet.

https://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on ice. Once thawed add assay buffer and proceed for homogenization.

- start with 10-100 mg of tissue
- add 500-1000 ul (406 volume) of assay buffer homogenize using dounce homogenizer 10-50 passes on ice
- If lysis is confirmed spin down and collect the supernatant as required.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Thank you for your reply.

I wish to detect the presence of antioxidant enzymes in the tissue (glutathione peroxidase and reductase, SOD and catalase most important) but also the quantification or activity of each enzyme is important. Essentially I will be comparing tissue from two groups (disease and no disease) and then examining the effect of two drugs:

The total study will be (n = about 6 to 8 tissues per group):

Disease (n=8), No disease (n=8)
Drug A: Group 1 disease (n=8), Group 2 no disease (n=8)
Drug B: Group 3 disease (n=8), Group 4 no disease (n=8)
Drug A + B: Group 5 disease (n=8), Group 6 no disease (n=8)

I appreciate your advice

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Answer

Thank you for contacting us.

Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use are website there are range of antibodies available as per your requirements.

The following kits I can recommend for assaying the activity of these enzyme;

https://www.abcam.com/glutathione-reductase-gr-assay-kit-ab83461.html

https://www.abcam.com/glutathione-peroxidase-assay-kit-ab102530.html

https://www.abcam.com/superoxide-dismutase-activity-colorimetric-assay-kit-ab65354.html

https://www.abcam.com/catalase-assay-kit-ab83464.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I am inquiring about your total antioxidant capacity assay kit or similar.

I have frozen placental tissue from rabbit experiments and I am wishing to examine markers of oxidative stress.
The most important markers for me are glutathione peroxidase and reductase, superoxide dismutase (SOD) and catalase.

Can you kindly advise me if any of your products are potentially of use and a quote for cost please. I am in Belgium.

Regards,

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Answer

Thank you for contacting us.

Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

I will be looking forward to hearing from you soon.

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