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Glutathione Reductase (GR) Assay Kit (ab83461)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (3)References (12)

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Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 40 min
  • Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.1 mU/ml

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Overview

  • Product name

    Glutathione Reductase (GR) Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay type

    Enzyme activity
  • Sensitivity

    > 0.1 mU/ml
  • Range

    0.1 mU/ml - 40 mU/ml
  • Assay time

    0h 40m
  • Product overview

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.

  • Notes

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 200 tests
    Catalase (lyophilized) Clear 1 x 1ng
    DTNB (lyophilized) Red 1 vial
    GR Assay Buffer NM 1 x 100ml
    GR Positive Control (10 mU; lyophilized) Green 1 vial
    GSSG (lyophilized) Yellow 1 vial
    Hydrogen peroxide Orange 1 x 1ml
    NADPH-GNERAT™ (lyophilized) Blue 2 vials
    TNB Standard (2.5 µmol) Brown 1 x 1ng
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Cell Signaling Kits
    • Coenzyme and Cofactor Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
  • Relevance

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
  • Cellular localization

    Cytoplasmic and Mitochondrial
  • Alternative names

    • EC 1.8.1.7
    • Glutathione reductase mitochondrial
    • GR
    • GSR
    • MGC78522
    see all

Associated products

    Images

    • Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)Cao, Zeyu et al., Scientific reports?vol. 7,1 16278., Fig 5, doi:10.1038/s41598-017-16446-7
      ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)

      Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)

      Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)

      Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Glutathione Reductase assay time line using ab83461.
    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      TNB Standard Curve using ab83461.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (12)

    Publishing research using ab83461? Please let us know so that we can cite the reference in this datasheet.

    ab83461 has been referenced in 12 publications.

    • Bauckneht M  et al. FDG uptake tracks the oxidative damage in diabetic skeletal muscle: An experimental study. Mol Metab 31:98-108 (2020). PubMed: 31918925
    • Lechowicz K  et al. Two Festuca Species-F. arundinacea and F. glaucescens-Differ in the Molecular Response to Drought, While Their Physiological Response Is Similar. Int J Mol Sci 21:N/A (2020). PubMed: 32365894
    • Al-Otaibi NAS  et al. Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives. Gigascience 8:N/A (2019). PubMed: 30535373
    • Al-Otaibi NAS  et al. Cryopreservation of Red Blood Cells. Methods Mol Biol 1916:233-238 (2019). PubMed: 30535700
    • Al-Otaibi NAS  et al. Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryopreservation. Methods Mol Biol 1916:239-247 (2019). PubMed: 30535701
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Inquiry: Hello, I am considering ordering Glutathione Reductase (GR) Assay Kit (ab83461) to study this enzyme activity. I have frozen rabbit placenta. Can you advise me of the details / protocol of preparing this tissue for this analysis (ie tissue extract), so that I can confirm my lab has the ability to do this. Do you have knowledge whether this has been done before in this type of tissue and do you anticipate any difficulties? Many thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    The procedure is given in protocol booklet.

    https://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

    Please thaw frozen tissue on ice. Once thawed add assay buffer and proceed for homogenization.

    - start with 10-100 mg of tissue
    - add 500-1000 ul (406 volume) of assay buffer homogenize using dounce homogenizer 10-50 passes on ice
    - If lysis is confirmed spin down and collect the supernatant as required.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Question

    Thank you for your reply.

    I wish to detect the presence of antioxidant enzymes in the tissue (glutathione peroxidase and reductase, SOD and catalase most important) but also the quantification or activity of each enzyme is important. Essentially I will be comparing tissue from two groups (disease and no disease) and then examining the effect of two drugs:

    The total study will be (n = about 6 to 8 tissues per group):

    Disease (n=8), No disease (n=8)
    Drug A: Group 1 disease (n=8), Group 2 no disease (n=8)
    Drug B: Group 3 disease (n=8), Group 4 no disease (n=8)
    Drug A + B: Group 5 disease (n=8), Group 6 no disease (n=8)

    I appreciate your advice

    Read More

    Abcam community

    Verified customer

    Asked on Nov 12 2012

    Answer

    Thank you for contacting us.

    Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use are website there are range of antibodies available as per your requirements.

    The following kits I can recommend for assaying the activity of these enzyme;

    https://www.abcam.com/glutathione-reductase-gr-assay-kit-ab83461.html

    https://www.abcam.com/glutathione-peroxidase-assay-kit-ab102530.html

    https://www.abcam.com/superoxide-dismutase-activity-colorimetric-assay-kit-ab65354.html

    https://www.abcam.com/catalase-assay-kit-ab83464.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 12 2012

    Question

    I am inquiring about your total antioxidant capacity assay kit or similar.

    I have frozen placental tissue from rabbit experiments and I am wishing to examine markers of oxidative stress.
    The most important markers for me are glutathione peroxidase and reductase, superoxide dismutase (SOD) and catalase.

    Can you kindly advise me if any of your products are potentially of use and a quote for cost please. I am in Belgium.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 12 2012

    Answer

    Thank you for contacting us.

    Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

    I will be looking forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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