Glutathione Reductase (GR) Assay Kit (ab83461)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mU/ml
Overview
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Product name
Glutathione Reductase (GR) Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Sensitivity
> 0.1 mU/ml -
Range
0.1 mU/ml - 40 mU/ml -
Assay time
0h 40m -
Product overview
Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Since Glutathione Reductase has significantly higher concentrations in cells (mM range) compared to Thioredoxin Reductase (µM range), we predict that ab83461 will detect mostly GR activity in samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K761 Glutathione Reductase Activity Colorimetric Assay Kit. K761-200 is the same size as the 200 test size of ab83461.
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
Related products
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 200 tests Catalase (lyophilized) Clear 1 x 1ng DTNB (lyophilized) Red 1 vial GR Assay Buffer NM 1 x 100ml GR Positive Control (10 mU; lyophilized) Green 1 vial GSSG (lyophilized) Yellow 1 vial Hydrogen peroxide Orange 1 x 1ml NADPH-GNERAT™ (lyophilized) Blue 2 vials TNB Standard (2.5 µmol) Brown 1 x 1ng -
Research areas
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Relevance
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. -
Cellular localization
Cytoplasmic and Mitochondrial -
Alternative names
- EC 1.8.1.7
- Glutathione reductase mitochondrial
- GR
see all
Images
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Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)Cao, Zeyu et al., Scientific reports?vol. 7,1 16278., Fig 5, doi:10.1038/s41598-017-16446-7ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.
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Functional Studies - Glutathione Reductase Assay Kit (ab83461)Glutathione Reductase assay time line using ab83461. -
Functional Studies - Glutathione Reductase Assay Kit (ab83461)TNB Standard Curve using ab83461.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab83461 has been referenced in 19 publications.
- Tang S et al. Herb Sanqi-Derived Compound K Alleviates Oxidative Stress in Cultured Human Melanocytes and Improves Oxidative-Stress-Related Leukoderma in Guinea Pigs. Cells 10:N/A (2021). PubMed: 34440826
- Bauckneht M et al. FDG uptake tracks the oxidative damage in diabetic skeletal muscle: An experimental study. Mol Metab 31:98-108 (2020). PubMed: 31918925
- Lechowicz K et al. Two Festuca Species-F. arundinacea and F. glaucescens-Differ in the Molecular Response to Drought, While Their Physiological Response Is Similar. Int J Mol Sci 21:N/A (2020). PubMed: 32365894
- Marini C et al. Mechanisms underlying the predictive power of high skeletal muscle uptake of FDG in amyotrophic lateral sclerosis. EJNMMI Res 10:76 (2020). PubMed: 32638178
- Villa-Bellosta R Dietary magnesium supplementation improves lifespan in a mouse model of progeria. EMBO Mol Med 12:e12423 (2020). PubMed: 32875720