Overview

  • Product name
    Glutathione Reductase (GR) Assay Kit
  • Detection method
    Colorimetric
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay type
    Enzyme activity
  • Sensitivity
    > 0.1 mU/ml
  • Range
    0.1 mU/ml - 40 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.

  • Notes

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform
    Microplate reader

Properties

Images

  • ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
  • Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Glutathione Reductase assay time line using ab83461.
  • TNB Standard Curve using ab83461.

Protocols

References

This product has been referenced in:
  • Szabó K  et al. Fenugreek (Trigonella Foenum-Graecum) Seed Flour and Diosgenin Preserve Endothelium-Dependent Arterial Relaxation in a Rat Model of Early-Stage Metabolic Syndrome. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29534453) »
  • de Pádua Lúcio K  et al. Anti-Inflammatory and Antioxidant Properties of Black Mulberry (Morus nigra L.) in a Model of LPS-Induced Sepsis. Oxid Med Cell Longev 2018:5048031 (2018). Read more (PubMed: 30524657) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Thank you for contacting us.

The procedure is given in protocol booklet.

https://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on ice. Once thawed add assay buffer and proceed for homogenization.

- start with 10-100 mg of tissue
- add 500-1000 ul (406 volume) of assay buffer homogenize using dounce homogenizer 10-50 passes on ice
- If lysis is confirmed spin down and collect the supernatant as required.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use are website there are range of antibodies available as per your requirements.

The following kits I can recommend for assaying the activity of these enzyme;

https://www.abcam.com/glutathione-reductase-gr-assay-kit-ab83461.html

https://www.abcam.com/glutathione-peroxidase-assay-kit-ab102530.html

https://www.abcam.com/superoxide-dismutase-activity-colorimetric-assay-kit-ab65354.html

https://www.abcam.com/catalase-assay-kit-ab83464.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

I will be looking forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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