Anti-GSTA1 antibody (ab53940)
Key features and details
- Goat polyclonal to GSTA1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Rabbit, Hamster, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GSTA1 antibody
See all GSTA1 primary antibodies -
Description
Goat polyclonal to GSTA1 -
Host species
Goat -
Specificity
The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk). -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Hamster, Human -
Immunogen
Full length native protein (purified) corresponding to Rat GSTA1.
Database link: P00502 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000.
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.
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Notes |
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IHC-P
1/1000. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). 5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.
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Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Domain
The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2938 Human
- Entrez Gene: 14857 Mouse
- Entrez Gene: 24421 Rat
- Omim: 138359 Human
- SwissProt: P08263 Human
- SwissProt: P13745 Mouse
- SwissProt: P00502 Rat
- Unigene: 446309 Human
see all -
Alternative names
- Glutathione S alkyltransferase A1 antibody
- Glutathione S aryltransferase A1 antibody
- Glutathione S transferase 2 antibody
see all
Images
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Lane 1. Molecular Weight Marker
Lane 2. GST α Standard
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature. -
A. Negative control (no antibody).
B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.
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ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab53940 has been referenced in 7 publications.
- Liu C et al. MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8. Cancer Cell Int 18:54 (2018). WB, IHC-P ; Human . PubMed: 29632436
- Liu H et al. Downregulation of Glutathione S-transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncol Lett 16:467-474 (2018). PubMed: 29928434
- Singh A et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). PubMed: 28289414
- Grootaert MO et al. Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy 11:2014-2032 (2015). WB ; Mouse . PubMed: 26391655
- Ochiai H et al. Glutathione S-transferase π complexes with and stimulates Na⁺,K⁺-ATPase. J Mol Recognit 26:32-7 (2013). PubMed: 23280615
- Volkova M et al. Activation of the aryl hydrocarbon receptor by doxorubicin mediates cytoprotective effects in the heart. Cardiovasc Res 90:305-14 (2011). WB ; Rat . PubMed: 21233252
- Azkargorta M et al. Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes. Mol Cell Proteomics 9:2184-94 (2010). WB ; Mouse . PubMed: 20573986