Anti-Glutathione S Transferase mu antibody (ab53942)


  • Product name
    Anti-Glutathione S Transferase mu antibody
    See all Glutathione S Transferase mu primary antibodies
  • Description
    Goat polyclonal to Glutathione S Transferase mu
  • Host species
  • Specificity
    ab53942 is specific for Glutathione S Transferase (GST) mu. It reacts with subtype 3-3 (M1) and subtype 4-4 (M2).
  • Tested applications
    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
  • Immunogen

    Full length native protein (purified) Rat Glutathione S Transferase mu.

  • Positive control
    • ICC/IF: HepG2 cells.



Our Abpromise guarantee covers the use of ab53942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 23741294
ICC/IF Use a concentration of 5 µg/ml.
WB 1/3000. Detects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).


  • Function
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Tissue specificity
    Liver (at protein level).
  • Sequence similarities
    Belongs to the GST superfamily. Mu family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione S alkyltransferase antibody
    • Glutathione S aralkyltransferase antibody
    • Glutathione S aryltransferase antibody
    • Glutathione S transferase M1 antibody
    • Glutathione S transferase mu 1 antibody
    • Glutathione S transferase mu antibody
    • Glutathione S-transferase Mu 1 antibody
    • GST class mu 1 antibody
    • GST class Mu antibody
    • GST class-mu 1 antibody
    • GST HB subunit 4 antibody
    • GST mu antibody
    • GST1 antibody
    • GSTM1 1 antibody
    • Gstm1 antibody
    • GSTM1-1 antibody
    • GSTM1_HUMAN antibody
    • GSTM1a 1a antibody
    • GSTM1a-1a antibody
    • GSTM1b 1b antibody
    • GSTM1b-1b antibody
    • GTH4 antibody
    • GTM1 antibody
    • H B antibody
    • HB subunit 4 antibody
    • MGC26563 antibody
    • MU 1 antibody
    • MU antibody
    • S (hydroxyalkyl)glutathione lyase antibody
    see all


  • ICC/IF image of ab53942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53942, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1. Molecular weight marker
    Lane 2. GST µ standard

    A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.


This product has been referenced in:
  • Shelton LM  et al. Integrated transcriptomic and proteomic analyses uncover regulatory roles of Nrf2 in the kidney. Kidney Int 88:1261-1273 (2015). WB ; Mouse . Read more (PubMed: 26422507) »
  • Klaus A  et al. Glutathione S-Transferases Interact with AMP-Activated Protein Kinase: Evidence for S-Glutathionylation and Activation In Vitro. PLoS One 8:e62497 (2013). IP ; Rat . Read more (PubMed: 23741294) »

See all 4 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Mouse Tissue lysate - whole (Brain tissue)
Brain tissue
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2014

I have found two antibodies for rabbit samples that may be effective for detecting two of the proteins you specified. Anti-GCLC ab80841 is raised against an immunogen sequence that is conserved 100% in the rabbit sequence listed in the UniProt entry fo...

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Thank you for your sample preparation protocol. It is standard and much as I expected. What I meant by "reduced" was whether you had included a reducing agent in the loading buffer, to reduce di-sulfide bonds. The GST apparently exists as a di-sulfide-...

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Thank you for bringing this to our attention. The predicted molecular weight of the GST precursor is 27 kDa. I am wondering if what you see is a dimer. Can you describe your samples and how you prepare them? Are they reduced? The following database ent...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - other (Liver)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 15 2010

Thank you for your patience in this matter. The originator of ab53942 (Glutathione S Transferase mu antibody) informed us that this product reacts with mu's subunit 3 and 4. I hope this helps. Please don't hesitate to contact us again if you hav...

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