Recombinant Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutathione S Transferase mu antibody [EPR1936(2)]
See all Glutathione S Transferase mu primary antibodies -
Description
Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu -
Host species
Rabbit -
Specificity
This is an Anti-GSTM1 antibody, but it's immunogen shares 85% homology with GSTM5. Cross-reactivity with GSTM5 has not been confirmed experimentally.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, SH-SY5Y and NIH/3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1936(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Tissue specificity
Liver (at protein level). -
Sequence similarities
Belongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2944 Human
- Entrez Gene: 14862 Mouse
- Entrez Gene: 24423 Rat
- Omim: 138350 Human
- SwissProt: P09488 Human
- SwissProt: P10649 Mouse
- SwissProt: P04905 Rat
- Unigene: 301961 Human
see all -
Alternative names
- Glutathione S alkyltransferase antibody
- Glutathione S aralkyltransferase antibody
- Glutathione S aryltransferase antibody
see all
Images
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All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + NIH/3T3 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + PC-12 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108524 has been referenced in 2 publications.
- Sciarretta F et al. Glutathione transferase P silencing promotes neuronal differentiation of retinal R28 cells. J Cell Physiol 234:15885-15897 (2019). PubMed: 30741416
- Kratzer I et al. Glutathione Conjugation at the Blood-CSF Barrier Efficiently Prevents Exposure of the Developing Brain Fluid Environment to Blood-Borne Reactive Electrophilic Substances. J Neurosci 38:3466-3479 (2018). PubMed: 29507144