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Tags & Cell Markers Fusion / Marker Proteins GST
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RecombinantRabMAb

Recombinant Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)

  • Datasheet
  • SDS
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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)
  • Immunocytochemistry/ Immunofluorescence - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)
  • Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8371(B)] to glutathione S transferase Omega 1/p28
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)]
    See all glutathione S transferase Omega 1/p28 primary antibodies
  • Description

    Rabbit monoclonal [EPR8371(B)] to glutathione S transferase Omega 1/p28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver tissue lysate, Jurkat and HeLa cell lysates; Jurkat cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as glutathione S transferase Omega 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8371(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GST

Associated products

  • Alternative Versions

    • Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] - BSA and Azide free (ab248395)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
    • Tissue specificity

      Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung.
    • Sequence similarities

      Belongs to the GST superfamily. Omega family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Cellular localization

      Cytoplasm > cytosol.
    • Target information above from: UniProt accession P78417 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9446 Human
      • Omim: 605482 Human
      • SwissProt: P78417 Human
      • Unigene: 190028 Human
      • Alternative names

        • AA407097 antibody
        • AI194287 antibody
        • AU018802 antibody
        • DKFZp686H13163 antibody
        • EC 2.5.1.18 antibody
        • Glutathione S transferase omega 1 antibody
        • Glutathione S-transferase omega 1-1 antibody
        • Glutathione S-transferase omega antibody
        • Glutathione S-transferase omega-1 antibody
        • Glutathione transferase omega 1 antibody
        • Glutathione-dependent dehydroascorbate reductase antibody
        • Glutathione-S-transferase like antibody
        • Glutathione-S-transferase omega 1 antibody
        • GSTO 1-1 antibody
        • GSTO-1 antibody
        • GSTO1 1 antibody
        • Gsto1 antibody
        • GSTO1_HUMAN antibody
        • GSTTLp28 antibody
        • GSTX antibody
        • Gtsttl antibody
        • MGC94845 antibody
        • MMA(V) reductase antibody
        • Monomethylarsonic acid reductase antibody
        • OTTHUMP00000020429 antibody
        • p28 antibody
        • S-(Phenacyl)glutathione reductase antibody
        • SPG-R antibody
        see all

      Images

      • Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)
        Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)
        All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262) at 1/1000 dilution

        Lane 1 : Human fetal liver tissue lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 28 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)
        Immunocytochemistry/ Immunofluorescence - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)

        Immunofluorescent staining of Jurkat cells labelling glutathione S transferase Omega 1/p28 with ab131262 at 1/50 dilution.

      • Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)
        Anti-glutathione S transferase Omega 1/p28 antibody [EPR8371(B)] (ab131262)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab131262? Please let us know so that we can cite the reference in this datasheet.

      ab131262 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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