Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535)

Overview

  • Product name

    Anti-Glutathione S Transferase pi antibody - C-terminal
    See all Glutathione S Transferase pi primary antibodies
  • Description

    Rabbit polyclonal to Glutathione S Transferase pi - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 171-201 of Human Glutathione S Transferase pi.

  • Positive control

    • Y79 cell line lysate; 293 cell lysate transiently transfected with the Glutathione S Transferase pi gene; Widr cells; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab135535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 23 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Sequence similarities

    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deafness X linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty acid ethyl ester synthase III antibody
    • Glutathione S transferase pi 1 antibody
    • Glutathione S-transferase P antibody
    • GST class-pi antibody
    • GST3 antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • GSTpi antibody
    • PI antibody
    see all

Images

  • Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535) at 1/50 dilution + Y79 cell line lysate at 35 µg

    Predicted band size: 23 kDa

  • All lanes : Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the Glutathione S Transferase pi gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 23 kDa

  • Flow cytometric analysis of widr cells labelling Glutathione S Transferase pi with ab135535 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • All lanes : Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the Glutathione S Transferase pi gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 23 kDa

  • Immunofluorescent analysis of HeLa cells labelling Glutathione S Transferase pi with ab135535 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

References

ab135535 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Human Cell (ES-derived hepatocytes)
Specification
ES-derived hepatocytes
Permeabilization
Yes - During blocking step, BSA is diluted in PBS/0.1% Tween.
Fixative
Methanol

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Submitted Aug 20 2013

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