Overview

  • Product name

    Glutathione Sepharose®
  • Product overview

    Contents:

    Supplied as a 50 % slurry in 20 % Ethanol / PBS; > 3 mg (10 µmol) glutathione per mL Sepharose® beads.

     

    Features:

    The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the overexpression of foreign genes in E. Coli and other expression systems. The GST-tagged protein can be easily purified from cell lysate or media by affinity chromatography using glutathione Sepharose® beads. Abcam’s Glutathione Sepharose® is designed for the purification of GST fusion proteins and other glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.

    These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.

    Store beads at 4°C.

    The beads may be damaged above 40°C.

    DO NOT FREEZE.

    Wash beads 3 times with 3x bead volume of desired buffer before use.

     

    Applications:

    Purification of GST-fusion proteins or other glutathione-binding proteins.

     

    Sepharose is a registered trademark of GE Healthcare

  • Notes

    Glutathione Sepharose® is prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked Sepharose® beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity, which could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel.

  • Tested applications

    Suitable for: Purificationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab193267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Purification of GST-fusion proteins or other glutathione-binding proteins.

References

ab193267 has not yet been referenced specifically in any publications.

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