Glutathione Sepharose® (ab193267)
Overview
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Product name
Glutathione Sepharose® -
Product overview
Contents:
Supplied as a 50 % slurry in 20 % Ethanol / PBS; > 3 mg (10 µmol) glutathione per mL Sepharose® beads.
Features:
The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the overexpression of foreign genes in E. Coli and other expression systems. The GST-tagged protein can be easily purified from cell lysate or media by affinity chromatography using glutathione Sepharose® beads. Abcam’s Glutathione Sepharose® is designed for the purification of GST fusion proteins and other glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of GST-fusion proteins or other glutathione-binding proteins.
Sepharose is a registered trademark of GE Healthcare
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6555 Glutathione Sepharose. 6555-10 is the same size as the 10 ml size of ab193267.
Glutathione Sepharose® is prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked Sepharose® beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity, which could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 10 ml 50 ml Glutathione Sepharose® 1 x 1ml 1 x 10ml 1 x 50ml -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Purification |
Use at an assay dependent concentration.
Purification of GST-fusion proteins or other glutathione-binding proteins. |
| Notes |
|---|
|
Purification
Use at an assay dependent concentration. Purification of GST-fusion proteins or other glutathione-binding proteins. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab193267 has been referenced in 2 publications.
- Wang W et al. Hemidesmosomes modulate force generation via focal adhesions. J Cell Biol 219:N/A (2020). PubMed: 31914171
- Dagliyan O et al. Engineering proteins for allosteric control by light or ligands. Nat Protoc 14:1863-1883 (2019). PubMed: 31076662