Anti-Glutathione Synthetase antibody (ab175263)
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Overview
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Product nameAnti-Glutathione Synthetase antibody
See all Glutathione Synthetase primary antibodies -
DescriptionRabbit polyclonal to Glutathione Synthetase
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cynomolgus monkey
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Immunogen
Recombinant full length protein corresponding to Human Glutathione Synthetase aa 1-474.
Sequence:MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPF TLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFT ARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISA SFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYG SPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQ DRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQ LAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQA IAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEK IEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLR TKAIEHADGGVAAGVAVLDNPYPV
Database link: P48637 -
Positive control
- 293T, HeLa and HT-1080 cell line extracts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/500 - 1/2000. Predicted molecular weight: 52 kDa. |
Target
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PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
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Involvement in diseaseDefects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. -
Sequence similaritiesBelongs to the eukaryotic GSH synthase family.
- Information by UniProt
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Database links
- Entrez Gene: 2937 Human
- Entrez Gene: 14854 Mouse
- Entrez Gene: 25458 Rat
- Omim: 601002 Human
- SwissProt: P48637 Human
- SwissProt: P51855 Mouse
- SwissProt: P46413 Rat
- Unigene: 82327 Human
see all -
Alternative names
- epididymis secretory sperm binding protein Li 64p antibody
- epididymis secretory sperm binding protein Li 88n antibody
- Glutathione synthase antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Synthetase antibody (ab175263)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175263. Blue DAPI for nuclear staining. -
Western blot - Anti-Glutathione Synthetase antibody (ab175263)All lanes : Anti-Glutathione Synthetase antibody (ab175263) at 1/500 dilution
Lane 1 : 293T cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : HT-1080 cell line lysate
Predicted band size: 52 kDa
Protocols
Datasheets and documents
References
ab175263 has not yet been referenced specifically in any publications.
