Anti-Glutathione Synthetase antibody (ab175263)
Key features and details
- Rabbit polyclonal to Glutathione Synthetase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glutathione Synthetase antibody
See all Glutathione Synthetase primary antibodies -
Description
Rabbit polyclonal to Glutathione Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Cynomolgus monkey -
Immunogen
Recombinant full length protein corresponding to Human Glutathione Synthetase aa 1-474.
Sequence:MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPF TLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFT ARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISA SFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYG SPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQ DRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQ LAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQA IAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEK IEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLR TKAIEHADGGVAAGVAVLDNPYPV
Database link: P48637 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa. |
Target
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Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. -
Involvement in disease
Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. -
Sequence similarities
Belongs to the eukaryotic GSH synthase family. - Information by UniProt
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Database links
- Entrez Gene: 2937 Human
- Omim: 601002 Human
- SwissProt: P48637 Human
- Unigene: 82327 Human
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Alternative names
- epididymis secretory sperm binding protein Li 64p antibody
- epididymis secretory sperm binding protein Li 88n antibody
- Glutathione synthase antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175263. Blue DAPI for nuclear staining.
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All lanes : Anti-Glutathione Synthetase antibody (ab175263) at 1/500 dilution
Lane 1 : 293T cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : HT-1080 cell line lysate
Predicted band size: 52 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175263 has not yet been referenced specifically in any publications.