Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6562] to Glutathione Synthetase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-Glutathione Synthetase antibody [EPR6562]
See all Glutathione Synthetase primary antibodies
DescriptionRabbit monoclonal [EPR6562] to Glutathione Synthetase
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Glutathione Synthetase. The exact sequence is proprietary.
- 293T, HeLa, SW480 and HT 1080 cell lysates; Human kidney tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesab124811 is not purified, it is provided in storage buffer.
Our Abpromise guarantee covers the use of ab124811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
Involvement in diseaseDefects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
Sequence similaritiesBelongs to the eukaryotic GSH synthase family.
- Information by UniProt
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- Glutathione synthase antibody
All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution
Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : HT 1080 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
ab124811, at 1/50 dilution staining Glutathione Synthetase in paraffin-embedded Human kidney tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124811 has been referenced in 3 publications.
- Nishizawa S et al. Low tumor glutathione level as a sensitivity marker for glutamate-cysteine ligase inhibitors. Oncol Lett 15:8735-8743 (2018). PubMed: 29928324
- Liu C et al. Diallyl Trisulfide Protects Motor Neurons from the Neurotoxic Protein TDP-43 via Activating Lysosomal Degradation and the Antioxidant Response. Neurochem Res 43:2304-2312 (2018). PubMed: 30317421
- Parsanathan R & Jain SK Hydrogen sulfide increases glutathione biosynthesis, and glucose uptake and utilisation in C2C12 mouse myotubes. Free Radic Res 52:288-303 (2018). PubMed: 29378451