Recombinant Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
![Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-450207-anti-glutathione-synthetase-antibody-epr6562-western-blot-hek-293t-hela-daudi-lysates.jpg "Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6562] to Glutathione Synthetase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Glutathione Synthetase antibody [EPR6562]
See all Glutathione Synthetase primary antibodies -
Description
Rabbit monoclonal [EPR6562] to Glutathione Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Glutathione Synthetase. The exact sequence is proprietary.
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Positive control
- 293T, HeLa, Daudi, SW480 and HT 1080 cell lysates; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
ab124811 is not purified, it is provided in storage buffer. -
Clonality
Monoclonal -
Clone number
EPR6562 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
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| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
Target
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Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. -
Involvement in disease
Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. -
Sequence similarities
Belongs to the eukaryotic GSH synthase family. - Information by UniProt
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Database links
- Entrez Gene: 2937 Human
- Entrez Gene: 14854 Mouse
- Entrez Gene: 25458 Rat
- Omim: 601002 Human
- SwissProt: P48637 Human
- SwissProt: P51855 Mouse
- SwissProt: P46413 Rat
- Unigene: 82327 Human
see all -
Alternative names
- epididymis secretory sperm binding protein Li 64p antibody
- epididymis secretory sperm binding protein Li 88n antibody
- Glutathione synthase antibody
see all
Images
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Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : GSS knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDaLanes 1- 4: Merged signal (red and green). Green - ab124811 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab124811 was shown to react with GSS in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266342 (knockout cell lysate ab257460) was used. Wild-type HEK-293T and GSS knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124811 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-138722-anti-glutathione-synthetase-antibody-epr6562-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)ab124811, at 1/50 dilution staining Glutathione Synthetase in paraffin-embedded Human kidney tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-138723-anti-glutathione-synthetase-antibody-epr6562-western-blot.jpg)
Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution
Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : HT 1080 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![OI-RD Scanning - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-138721-anti-glutathione-synthetase-antibody-epr6562.jpg)
OI-RD Scanning - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) -
![Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-4-benefits-of-recombinant-antibodies.png)
Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab124811 has been referenced in 4 publications.
- Yang Y et al. RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer. Cancer Cell Int 20:587 (2020). PubMed: 33372599
- Nishizawa S et al. Low tumor glutathione level as a sensitivity marker for glutamate-cysteine ligase inhibitors. Oncol Lett 15:8735-8743 (2018). PubMed: 29928324
- Liu C et al. Diallyl Trisulfide Protects Motor Neurons from the Neurotoxic Protein TDP-43 via Activating Lysosomal Degradation and the Antioxidant Response. Neurochem Res 43:2304-2312 (2018). PubMed: 30317421
- Parsanathan R & Jain SK Hydrogen sulfide increases glutathione biosynthesis, and glucose uptake and utilisation in C2C12 mouse myotubes. Free Radic Res 52:288-303 (2018). PubMed: 29378451