Recombinant
RabMAb

Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)

Abreviews (5) Submit a Question

Overview

  • Product name
    Anti-Glutathione Synthetase antibody [EPR6562]
    See all Glutathione Synthetase primary antibodies
  • Description
    Rabbit monoclonal [EPR6562] to Glutathione Synthetase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glutathione Synthetase. The exact sequence is proprietary.

  • Positive control
    • 293T, HeLa, SW480 and HT 1080 cell lysates; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Pathway
      Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
    • Involvement in disease
      Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
      Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
    • Sequence similarities
      Belongs to the eukaryotic GSH synthase family.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • epididymis secretory sperm binding protein Li 64p antibody
      • epididymis secretory sperm binding protein Li 88n antibody
      • Glutathione synthase antibody
      • Glutathione synthetase antibody
      • GSH S antibody
      • GSH synthetase antibody
      • GSH-S antibody
      • GSHB_HUMAN antibody
      • GSHS antibody
      • GSS antibody
      • HEL-S-64p antibody
      • HEL-S-88n antibody
      • MGC14098 antibody
      • OTTHUMP00000030711 antibody
      see all

    Images

    • Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
      Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
      All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HT 1080 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
      ab124811, at 1/50 dilution staining Glutathione Synthetase in paraffin-embedded Human kidney tissue, by Immunohistochemistry.
    • Other - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
      Other - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124811 has not yet been referenced specifically in any publications.

    Publishing research using ab124811? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

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    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutathione Synthetase antibody [EPR6562]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (MDA-MB-468 tumor taken out of Balb/C nude mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    MDA-MB-468 tumor taken out of Balb/C nude mice
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
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    Submitted Jun 22 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutathione Synthetase antibody [EPR6562]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U937 cells)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    U937 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
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    Submitted May 24 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutathione Synthetase antibody [EPR6562]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (B16 cells)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    B16 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
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    Submitted May 24 2018

    Western blot abreview for Anti-Glutathione Synthetase antibody [EPR6562]

     Inconclusive
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (B16 tumor lysate)
    Gel Running Conditions
    Reduced Denaturing (4-20% TG gels)
    Loading amount
    50 µg
    Specification
    B16 tumor lysate
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Submitted Apr 10 2018

    Western blot abreview for Anti-Glutathione Synthetase antibody [EPR6562]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin)
    Loading amount
    50 µg
    Specification
    THP1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Verified customer

    Submitted Feb 21 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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