Recombinant Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
![Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-478730-anti-glutathione-synthetase-antibody-epr6562-westernblot-human.jpg "Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6562] to Glutathione Synthetase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutathione Synthetase antibody [EPR6562]
See all Glutathione Synthetase primary antibodies -
Description
Rabbit monoclonal [EPR6562] to Glutathione Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Glutathione Synthetase. The exact sequence is proprietary.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab124811 is not purified, it is provided in storage buffer. -
Clonality
Monoclonal -
Clone number
EPR6562 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IHC-P | (1) |
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) For unpurified use at dilution between 1/50 and 1/100 |
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IHC-P
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) For unpurified use at dilution between 1/50 and 1/100 |
Target
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Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. -
Involvement in disease
Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. -
Sequence similarities
Belongs to the eukaryotic GSH synthase family. - Information by UniProt
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Database links
- Entrez Gene: 2937 Human
- Omim: 601002 Human
- SwissProt: P48637 Human
- Unigene: 82327 Human
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Alternative names
- epididymis secretory sperm binding protein Li 64p antibody
- epididymis secretory sperm binding protein Li 88n antibody
- Glutathione synthase antibody
see all
Images
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Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-480066-anti-glutathione-synthetase-antibody-epr6562-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling Glutathione Synthetase with Purified ab124811 at 1:8000 (0.11 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-480065-anti-glutathione-synthetase-antibody-epr6562-immunohistochemistry-hepatocellular-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Glutathione Synthetase with Purified ab124811 at 1:8000 (0.11 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-450207-anti-glutathione-synthetase-antibody-epr6562-western-blot-hek-293t-hela-daudi-lysates.jpg)
Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : GSS knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDaLanes 1- 4: Merged signal (red and green). Green - ab124811 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab124811 was shown to react with GSS in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266342 (knockout cell lysate ab257460) was used. Wild-type HEK-293T and GSS knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124811 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)](https://www.abcam.com/ps/products/124/ab124811/Images/ab124811-4-benefits-of-recombinant-antibodies.png)
Anti-Glutathione Synthetase antibody [EPR6562] (ab124811)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab124811 has been referenced in 6 publications.
- Tang Z et al. Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin. Clin Transl Med 11:e390 (2021). PubMed: 33931967
- Yang Y et al. RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer. Cancer Cell Int 20:587 (2020). PubMed: 33372599
- Nishizawa S et al. Low tumor glutathione level as a sensitivity marker for glutamate-cysteine ligase inhibitors. Oncol Lett 15:8735-8743 (2018). PubMed: 29928324
- Liu C et al. Diallyl Trisulfide Protects Motor Neurons from the Neurotoxic Protein TDP-43 via Activating Lysosomal Degradation and the Antioxidant Response. Neurochem Res 43:2304-2312 (2018). PubMed: 30317421
- Parsanathan R & Jain SK Hydrogen sulfide increases glutathione biosynthesis, and glucose uptake and utilisation in C2C12 mouse myotubes. Free Radic Res 52:288-303 (2018). PubMed: 29378451
- Wang C et al. Acetaminophen attenuates glomerulosclerosis in obese Zucker rats via reactive oxygen species/p38MAPK signaling pathways. Free Radic Biol Med 81:47-57 (2015). PubMed: 25614458