Recombinant
RabMAb

Recombinant Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)

Overview

  • Product name

    Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free
    See all Glutathione Synthetase primary antibodies
  • Description

    Rabbit monoclonal [EPR6563] to Glutathione Synthetase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glutathione Synthetase aa 350-450 (internal sequence). The exact sequence is proprietary.
    (Peptide available as ab211544)

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab236062 is the carrier-free version of ab133592. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236062 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.Can be blocked with Glutathione Synthetase peptide (ab211544).

Target

  • Pathway

    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
  • Involvement in disease

    Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
    Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
  • Sequence similarities

    Belongs to the eukaryotic GSH synthase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • epididymis secretory sperm binding protein Li 64p antibody
    • epididymis secretory sperm binding protein Li 88n antibody
    • Glutathione synthase antibody
    • Glutathione synthetase antibody
    • GSH S antibody
    • GSH synthetase antibody
    • GSH-S antibody
    • GSHB_HUMAN antibody
    • GSHS antibody
    • GSS antibody
    • HEL-S-64p antibody
    • HEL-S-88n antibody
    • MGC14098 antibody
    • OTTHUMP00000030711 antibody
    see all

Images

  • Overlay histogram showing Jurkat cells stained with ab133592 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133592, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133592).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133592).

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Glutathione Synthetase with ab133592 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133592).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab236062 has not yet been referenced specifically in any publications.

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