Gly-Phe beta-naphthylamide, Cathepsin C substrate (ab145914)
- Datasheet
- References
Overview
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Product name
Gly-Phe beta-naphthylamide, Cathepsin C substrate -
Description
Cathepsin C substrate -
Alternative names
- Gly-L-Phe-2-NNap
- gly-phe 2-naphthylamide
- Gly-Phe beta-naphthylamide
see all -
Biological description
Substrate for the lysosomal cysteine protease Cathepsin C. Accumulates within the lysosome. Hydrolysis by cathepsin C degrades Gly-Phe-β-naphthylamide into fragments that do not easily diffuse through the lysosomal membrane, leading to a loss of lysosome membrane integrity. Used to study intralysosomal hydrolysis, lysosomal membrane permeability, and the function of cathepsin C. Inhibits the cathepsin-dependent activation of caspase-8.
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Purity
> 95% -
CAS Number
21438-66-4 -
Chemical structure
Properties
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Chemical name
Glycylphenylalanine 2-naphthylamide -
Molecular weight
347.41 -
Molecular formula
C21H21N3O2 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
References
This product has been referenced in:
- Qiu GF et al. Expression and purification of active recombinant cathepsin C (dipeptidyl aminopeptidase I) of kuruma prawn Marsupenaeus japonicus in insect cells. J Biomed Biotechnol 2009:746289 (2009). Read more (PubMed: 19707514) »
- Baumgartner HK et al. Caspase-8-mediated apoptosis induced by oxidative stress is independent of the intrinsic pathway and dependent on cathepsins. Am J Physiol Gastrointest Liver Physiol 293:G296-307 (2007). Read more (PubMed: 17431216) »
- Doughty MJ & Gruenstein EI Chloride-insensitive, glycine-phenylalanine-naphthylamide hydrolysis at neutral pH in human skin fibroblasts. Biochem Cell Biol 64:772-81 (1986). Read more (PubMed: 3768167) »
- Jadot M et al. Intralysosomal hydrolysis of glycyl-L-phenylalanine 2-naphthylamide. Biochem J 219:965-70 (1984). Read more (PubMed: 6743255) »
