Overview

  • Product name
    Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric)
    See all GAPDH kits
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Enzyme activity
  • Assay time
    0h 20m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric) (ab204732) provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 µU/mL in a variety of samples.


    GAPDH assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with microplate reader for 10 min every 2-3 min

  • Notes

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.

  • Platform
    Microplate reader

Properties

Associated products

Images

  • Typical NADH Standard Curve.

  • GAPDH activity in the Positive Control, 3T3 cells lysate and MDA-MB-468 cells lysate.

  • GAPDH specific activity calculated from 3T3 cell lysate (11.1 µg protein), and MDA-MB-468 cell lysate (2.76 µg protein).

Protocols

References

This product has been referenced in:
  • Ebner P  et al. Lantibiotic production is a burden for the producing staphylococci. Sci Rep 8:7471 (2018). Read more (PubMed: 29749386) »
  • Tallima H  et al. Protective immune responses against Schistosoma mansoni infection by immunization with functionally active gut-derived cysteine peptidases alone and in combination with glyceraldehyde 3-phosphate dehydrogenase. PLoS Negl Trop Dis 11:e0005443 (2017). Read more (PubMed: 28346516) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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