Key features and details
- Rabbit polyclonal to Glycerate kinase - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Glycerate kinase antibody - C-terminal
See all Glycerate kinase primary antibodies
DescriptionRabbit polyclonal to Glycerate kinase - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey
Recombinant fragment corresponding to Human Glycerate kinase aa 284-523 (C terminal).
VKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVL SAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQI PDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAE LRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATF LAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR
Database link: Q8IVS8-1
- WB: NIH/3T3 whole cell lysate; Mouse liver, lung and kidney tissue lysates; Rat lung tissue lysate. IHC-P: Human lung and liver tissues.
Previously labelled as GLYCTK.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 48,55 kDa.|
Tissue specificityWidely expressed.
Involvement in diseaseDefects in GLYCTK are the cause of D-glyceric aciduria (D-GA) [MIM:220120]. D-GA is a rare metabolic disease characterized by chronic metabolic acidosis and a highly variable clinical phenotype. Clinical features range from an encephalopathic presentation with seizures, microcephaly, severe mental retardation and early death, to milder manifestations with only speech delay or even normal development.
Sequence similaritiesBelongs to the glycerate kinase type-2 family.
Cellular localizationCytoplasm and Cytoplasm. Mitochondrion.
- Information by UniProt
- CG9886 like antibody
- GLCTK_HUMAN antibody
- Glycerate kinase antibody
All lanes : Anti-Glycerate kinase antibody - C-terminal (ab225936) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat lung tissue lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 48,55 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Paraffin-embedded human lung tissue stained for Glycerate kinase using ab225936 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for Glycerate kinase using ab225936 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225936 has not yet been referenced specifically in any publications.