Product nameAnti-Glycerate kinase antibody - C-terminal
See all Glycerate kinase primary antibodies
DescriptionRabbit polyclonal to Glycerate kinase - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey
Recombinant fragment corresponding to Human Glycerate kinase aa 284-523 (C terminal).
VKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVL SAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQI PDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAE LRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATF LAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR
Database link: Q8IVS8-1
- WB: NIH/3T3 whole cell lysate; Mouse liver, lung and kidney tissue lysates; Rat lung tissue lysate. IHC-P: Human lung and liver tissues.
Previously labelled as GLYCTK.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 48,55 kDa.|
Tissue specificityWidely expressed.
Involvement in diseaseDefects in GLYCTK are the cause of D-glyceric aciduria (D-GA) [MIM:220120]. D-GA is a rare metabolic disease characterized by chronic metabolic acidosis and a highly variable clinical phenotype. Clinical features range from an encephalopathic presentation with seizures, microcephaly, severe mental retardation and early death, to milder manifestations with only speech delay or even normal development.
Sequence similaritiesBelongs to the glycerate kinase type-2 family.
Cellular localizationCytoplasm and Cytoplasm. Mitochondrion.
- Information by UniProt
- CG9886 like antibody
- GLCTK_HUMAN antibody
- Glycerate kinase antibody
All lanes : Anti-Glycerate kinase antibody - C-terminal (ab225936) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat lung tissue lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 48,55 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Paraffin-embedded human lung tissue stained for Glycerate kinase using ab225936 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for Glycerate kinase using ab225936 at 1/100 dilution in immunohistochemical analysis.
ab225936 has not yet been referenced specifically in any publications.