Anti-Glycerol 3 Phosphate Dehydrogenase antibody - N-terminal (ab175961)

Overview

  • Product name
    Anti-Glycerol 3 Phosphate Dehydrogenase antibody - N-terminal
    See all Glycerol 3 Phosphate Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Glycerol 3 Phosphate Dehydrogenase - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Glycerol 3 Phosphate Dehydrogenase aa 35-65 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P21695

  • Positive control
    • Mouse brain lysate, Human brain tissue, HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI747587 antibody
    • cytoplasmic antibody
    • EC 1.1.1.8 antibody
    • FLJ26652 antibody
    • G3PD antibody
    • Gdc-1 antibody
    • Gdc1 antibody
    • Gdp1 antibody
    • Glycerol 3 phosphate dehydrogenase 1 antibody
    • Glycerol 3 phosphate dehydrogenase cytosolic antibody
    • Glycerol 3 phosphate dehydrogenase soluble antibody
    • Glycerol-3-phosphate dehydrogenase [NAD+] antibody
    • Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic antibody
    • Glycerol-3-phosphate dehydrogenase 1 (soluble) antibody
    • Glycerol-3-phosphate dehydrogenase antibody
    • Glycerol-3-phosphate dehydrogenase, soluble antibody
    • Glycerphosphate dehydrogenase antibody
    • GPD-C antibody
    • gpd1 antibody
    • Gpd1 protein antibody
    • Gpd3 antibody
    • GPDA_HUMAN antibody
    • Gpdc antibody
    • GPDH antibody
    • GPDH-C antibody
    • Gpdhc antibody
    • HTGTI antibody
    • KIAA4010 antibody
    • MGC93453 antibody
    • MGPD antibody
    • mKIAA4010 antibody
    see all

Images

  • Anti-Glycerol 3 Phosphate Dehydrogenase antibody - N-terminal (ab175961) at 1/1000 dilution + mouse brain lysate at 35 µg

    Predicted band size: 38 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling Glycerol 3 Phosphate Dehydrogenase using ab175961 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometry analysis of HepG2 cells labeling Glycerol 3 Phosphate Dehydrogenase (green, bottom histogram), using ab175961 at a 1/10 dilution, and negative control cells (blue, top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175961 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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