Overview

  • Product name
    Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Enzyme activity (quantitative)
  • Sensitivity
    < 1 mU/well
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways.

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    GPDH Assay Buffer 1 x 27ml
    GPDH Positive Control 1 vial
    GPDH Probe 1 vial
    GPDH Substrate 1 vial
    NADH Standard 1 vial
  • Research areas
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
    • GPD-C
    • GPDH-C

Images

  • NADH measured in cell lysates showing quantity (nmol) per 1 mln cells after 30 min incubation time.

    Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 2-18 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Measurement of Glycerol-3-Phosphate Dehydrogenase activity in 3T3-L1 pre- adipocyte (60 µg) and differentiated 3T3-L1 adipocytes (60 µg) using Glycerol-3-Phosphate Dehydrogenase activity (GPDH) Assay Kit (Colorimetric) (ab174095). This is example data only.

Protocols

References

This product has been referenced in:
  • Farr SA  et al. Metformin Improves Learning and Memory in the SAMP8 Mouse Model of Alzheimer's Disease. J Alzheimers Dis 68:1699-1710 (2019). Read more (PubMed: 30958364) »
  • Edmunds LR  et al. c-Myc and AMPK Control Cellular Energy Levels by Cooperatively Regulating Mitochondrial Structure and Function. PLoS One 10:e0134049 (2015). Read more (PubMed: 26230505) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174095.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up