Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (ab174094)

Overview

  • Product name
    Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Quantitative
  • Sensitivity
    < 2 nmol/well
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect G3P less than 2 nmol/well and can be used for a variety of sample types.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.

  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    G3P Assay Buffer 1 x 25ml
    G3P Enzyme Mix (Lyophilized) 1 vial
    G3P Probe 1 vial
    G3P Standard (Lyophilized) 1 vial
  • Research areas
  • Relevance
    Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol- 3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.
  • Alternative names
    • G3P
    • glycerol 3 phosphate
    • glycerol-3-phosphate

Images

  • This is example data only.

  • Measurement of G3P in rat liver (100 µg), 3T3-L1 (40 µg) and Hela (50 µg) lysate. This is example data.

Protocols

References

ab174094 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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