Key features and details
- Rabbit polyclonal to Glycerol kinase
- Suitable for: ELISA, WB
- Reacts with: Escherichia coli
- Isotype: IgG
Product nameAnti-Glycerol kinase antibody
See all Glycerol kinase primary antibodies
DescriptionRabbit polyclonal to Glycerol kinase
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Escherichia coli
Recombinant full length protein corresponding to Escherichia coli Glycerol kinase aa 2-502.
TEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPM EIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNA IVWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEG SRERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWD DKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGIAGDQQAAL FGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNY ALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTG LGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSG IRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLA VGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHD E
Database link: P0A6F3
- WB: DH5a whole cell lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab193521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/5000. Predicted molecular weight: 57 kDa.|
FunctionKey enzyme in the regulation of glycerol uptake and metabolism.
Tissue specificityHighly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
PathwayPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Involvement in diseaseDefects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
Sequence similaritiesBelongs to the FGGY kinase family.
Cellular localizationMitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
- Information by UniProt
- ATP glycerol 3 phosphotransferase antibody
- ATP:glycerol 3 phosphotransferase antibody
- ATP:glycerol 3-phosphotransferase antibody
Anti-Glycerol kinase antibody (ab193521) at 2 µg/ml + DH5a whole cell lysate
Polyclonal goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
All lanes : Anti-Glycerol kinase antibody (ab193521) at 3 µg/ml
Lane 1 : Rosseta bacteria lysate at 80 µg
Lane 2 : Rosseta bacteria lysate at 40 µg
Lane 3 : Rosseta bacteria lysate at 20 µg
Lane 4 : Rosseta bacteria lysate at 10 µg
All lanes : Goat polyclonal to rabbit IgG at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
ab193521 has not yet been referenced specifically in any publications.