Overview

  • Product name

    Anti-Glycerol kinase antibody
    See all Glycerol kinase primary antibodies
  • Description

    Rabbit polyclonal to Glycerol kinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee
  • Immunogen

    Recombinant fragment within Human Glycerol kinase (internal sequence). The exact sequence is proprietary.
    Database link: P32189

  • Positive control

    • WB: HepG2 whole cell lysate. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Key enzyme in the regulation of glycerol uptake and metabolism.
  • Tissue specificity

    Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
  • Pathway

    Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
  • Involvement in disease

    Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
  • Sequence similarities

    Belongs to the FGGY kinase family.
  • Cellular localization

    Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP glycerol 3 phosphotransferase antibody
    • ATP:glycerol 3 phosphotransferase antibody
    • ATP:glycerol 3-phosphotransferase antibody
    • D930012N15Rik antibody
    • GK antibody
    • GK1 antibody
    • GKD antibody
    • GLPK_HUMAN antibody
    • Glycerokinase antibody
    • Glycerol kinase antibody
    • OTTHUMP00000023108 antibody
    • OTTHUMP00000023109 antibody
    • OTTHUMP00000215321 antibody
    see all

Images

  • Anti-Glycerol kinase antibody (ab228615) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS-PAGE gel.

  • Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Glycerol kinase (green) using ab228615 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

References

ab228615 has not yet been referenced specifically in any publications.

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