Key features and details
- Rabbit polyclonal to Glycerol kinase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glycerol kinase antibody
See all Glycerol kinase primary antibodies
DescriptionRabbit polyclonal to Glycerol kinase
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee
Recombinant fragment within Human Glycerol kinase (internal sequence). The exact sequence is proprietary.
Database link: P32189
- WB: HepG2 whole cell lysate. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab228615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionKey enzyme in the regulation of glycerol uptake and metabolism.
Tissue specificityHighly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
PathwayPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Involvement in diseaseDefects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
Sequence similaritiesBelongs to the FGGY kinase family.
Cellular localizationMitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
- Information by UniProt
- ATP glycerol 3 phosphotransferase antibody
- ATP:glycerol 3 phosphotransferase antibody
- ATP:glycerol 3-phosphotransferase antibody
Anti-Glycerol kinase antibody (ab228615) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS-PAGE gel.
Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Glycerol kinase (green) using ab228615 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228615 has not yet been referenced specifically in any publications.