Recombinant
RabMAb

Recombinant Anti-Glycerol kinase antibody [EPR6567] (ab126599)

Overview

  • Product name

    Anti-Glycerol kinase antibody [EPR6567]
    See all Glycerol kinase primary antibodies
  • Description

    Rabbit monoclonal [EPR6567] to Glycerol kinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glycerol kinase aa 450-550. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse adipose tissue lysate. Fetal liver and HepG2 whole cell lysate (ab7900). FC: Permeabilized HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Key enzyme in the regulation of glycerol uptake and metabolism.
    • Tissue specificity

      Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
    • Pathway

      Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
    • Involvement in disease

      Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
    • Sequence similarities

      Belongs to the FGGY kinase family.
    • Cellular localization

      Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3-phosphotransferase antibody
      • D930012N15Rik antibody
      • GK antibody
      • GK1 antibody
      • GKD antibody
      • GLPK_HUMAN antibody
      • Glycerokinase antibody
      • Glycerol kinase antibody
      • OTTHUMP00000023108 antibody
      • OTTHUMP00000023109 antibody
      • OTTHUMP00000215321 antibody
      see all

    Images

    • All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution

      All lanes : Mouse adipose tissue lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : HRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 61 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 5 minutes

      See Abreview

    • All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 61 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?

    • ab126599, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by Immunofluorescence (red). A rabbit IgG is used as a negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Miao L  et al. Glycerol kinase interacts with nuclear receptor NR4A1 and regulates glucose metabolism in the liver. FASEB J 33:6736-6747 (2019). Read more (PubMed: 30821173) »
    • Kang M  et al. Improved systemic metabolism and adipocyte biology in miR-150 knockout mice. Metabolism N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 29352962) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Mouse Tissue lysate - whole (Adipose tissue lysate)
    Specification
    Adipose tissue lysate
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Aug 02 2013

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