Key features and details
- Rabbit polyclonal to Glycine decarboxylase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glycine decarboxylase antibody
See all Glycine decarboxylase primary antibodies
DescriptionRabbit polyclonal to Glycine decarboxylase
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human Glycine decarboxylase aa 492-693.
Database link: NP_000161
- HepG2 cells and lysate
Previously labelled as GLDC.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 113 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionThe glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.
Involvement in diseaseDefects in GLDC are a cause of non-ketotic hyperglycinemia (NKH) [MIM:605899]; also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms.
Sequence similaritiesBelongs to the gcvP family.
- Information by UniProt
- GCE antibody
- GCSP antibody
- GCSP_HUMAN antibody
All lanes : Anti-Glycine decarboxylase antibody (ab97625) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa
ab97625, at a 1/200 dilution, staining Glycine decarboxylase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower image was co-stained with Hoechst 33342.
ab97625 has been referenced in 4 publications.
- Zhou J et al. Identification and characterization of GLDC as host susceptibility gene to severe influenza. EMBO Mol Med 11:N/A (2019). PubMed: 30498026
- Li S et al. Astrocytes in primary cultures express serine racemase, synthesize d-serine and acquire A1 reactive astrocyte features. Biochem Pharmacol 151:245-251 (2018). PubMed: 29305854
- Swanson MA et al. Biochemical and molecular predictors for prognosis in nonketotic hyperglycinemia. Ann Neurol 78:606-18 (2015). PubMed: 26179960
- Kim SK et al. Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes. PLoS One 9:e101004 (2014). WB ; Human . PubMed: 24979213