Key features and details
- Rabbit polyclonal to Glycine Receptor alpha 1 + alpha 2
- Suitable for: IHC-Fr, WB, ICC/IF, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Glycine Receptor alpha 1 + alpha 2 antibody
DescriptionRabbit polyclonal to Glycine Receptor alpha 1 + alpha 2
SpecificityThis product recognises an epitope within the N-terminal region of glycine receptor alpha 1 and alpha 2 subunits.
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (N-terminal) (Rat).
- Rat brain lysate, rat spinal cord lysate.
General notesFor long term storage the addition of 0.09% sodium azide is recommended.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab23809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 48 kDa.|
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/1000 for overnight incubation on rat cells (see Abreview submitted by Johana Trojanova).|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21215254|
RelevanceGlycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Cellular localizationCell Membrane
- GLRA1 antibody
- GLRA2 antibody
- Glycine receptor 48 kDa subunit antibody
ab23809 at a 1/1000 dilution staining rat brain stem cells(medial nucleus of trapezoid body) by ICC/IF. The cells were fixed with paraformaldehyde and incubated with the antibody for 18 hours. An Alexa Fluor ® 488 donkey anti-rabbit antibody was used as the secondary.
ab23809 staining Glycin Receptor alpha1 + alpha 2 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/400 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
ab23809 has been referenced in 11 publications.
- Ren M et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . PubMed: 27112667
- Yoo YB et al. Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice. Mol Med Rep 12:7927-32 (2015). IHC . PubMed: 26498980
- Srivastava P et al. Differential alterations in the expression of neurotransmitter receptors in inner retina following loss of photoreceptors in rd1 mouse. PLoS One 10:e0123896 (2015). PubMed: 25835503
- Maskey D et al. Alteration of glycine receptor immunoreactivity in the auditory brainstem of mice following three months of exposure to radiofrequency radiation at SAR 4.0 W/kg. Int J Mol Med 34:409-19 (2014). IHC-FoFr ; Mouse . PubMed: 24866721
- Akaike Y et al. HuR regulates alternative splicing of the TRA2ß gene in human colon cancer cells under oxidative stress. Mol Cell Biol 34:2857-73 (2014). PubMed: 24865968