The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 48 kDa.
Use at an assay dependent concentration. Used at a dilution of 1/1000 for overnight incubation on rat cells (see Abreview submitted by Johana Trojanova).
Use at an assay dependent concentration. PubMed: 21215254
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Immunocytochemistry/ Immunofluorescence - Anti-Glycine Receptor alpha 1 + alpha 2 antibody (ab23809)This image is courtesy of an Abreview submitted by Ms Johana Trojanova
ab23809 at a 1/1000 dilution staining rat brain stem cells(medial nucleus of trapezoid body) by ICC/IF. The cells were fixed with paraformaldehyde and incubated with the antibody for 18 hours. An Alexa Fluor ® 488 donkey anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Frozen sections) - Anti-Glycine Receptor alpha 1 + alpha 2 antibody (ab23809)This image is courtesy of an Abreview submitted by Kevin Jin
ab23809 staining Glycin Receptor alpha1 + alpha 2 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/400 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
Ren M et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep6:24953 (2016).
Read more (PubMed: 27112667) »
Yoo YB et al. Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice. Mol Med Rep12:7927-32 (2015).
Read more (PubMed: 26498980) »