Glycogen Assay Kit (ab65620)
- Datasheet
- Specific References (19)
- Protocols
Overview
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Product nameGlycogen Assay Kit
See all Glycogen kits -
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue
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Assay typeQuantitative
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Sensitivity> 0.4 µg/ml
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Range0.4 µg/ml - 2000 µg/ml
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Assay time0h 40m
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Product overview
Glycogen Assay Kit (ab65620) is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.
If your sample is likely to contain reducing substances, we recommend using Glycogen Assay Kit II (ab169558), as reducing substances may interfere with the assay detection method.
Visit our FAQs page for tips and troubleshooting.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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Notes
Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in alpha-1,4 linkage, with branching via alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Storage bufferPreservative: None
Constituents: 0.1% Triton-X-100, DMSO -
Components Identifier 100 tests Development Buffer WM 1 x 25ml Development Enzyme Mix (lyophilized) Green 1 vial Glycogen Standard (2.0 mg/ml) Yellow 1 x 100µl Hydrolysis Buffer NM 1 x 25ml Hydrolysis Enzyme Mix (lyophilized) Blue 1 vial OxiRed Probe Red 1 x 200µl -
Research areas
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RelevanceGlycogen is the primary short term energy storage molecule in animals. It is synthesized primarily in the liver and muscle. Glycogen is a highly branched polymer of glucose molecules, connected with an alpha-1,4 linkage, branching via an alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.
Images
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Functional studies - ab65620Image from Roy Choudhury G et al., PLoS One 10(4), Fig 6c. Doi: 10.1371/journal.pone.0123096.Total glycogen levels in C576bL6 mice astrocytes were determined by using Glycogen assay kit (ab65620). At 24 hours following OGD-reoxygenation, astrocytes had less glycogen levels compared to normoxia control. Astrocytes treated with Methylene blue (MB) showed a higher glycogen content compared to non-MB treated, OGD-reoxygenation astrocytes. * p < 0.05; ## p < 0.001 Vs. OGD-reoxygenation control / 0 μM MB.
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Functional Studies - Glycogen Assay KitExample of fluorometric standard curve using Glycogen Assay Kit (ab65620).
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Functional Studies - Glycogen Assay Kit (ab65620)Measurement of glycogen in various mouse tissues using Glycogen Assay Kit (ab65620).
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Functional Studies - Glycogen Assay Kit (ab65620)Glycogen concentration measured in MBA-MB-231 cells (human breast adenocarcinoma cell line). 106 cells were prepared following protocol instructions, and several dilutions were measured using fluorometric detection.
Protocols
References
This product has been referenced in:
- Sobieski C et al. Ambient but not local lactate underlies neuronal tolerance to prolonged glucose deprivation. PLoS One 13:e0195520 (2018). Read more (PubMed: 29617444) »
- Guo J et al. Liraglutide reduces hepatic glucolipotoxicity-induced liver cell apoptosis through NRF2 signaling in Zucker diabetic fatty rats. Mol Med Rep 17:8316-8324 (2018). Read more (PubMed: 29693190) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"