• Product name
    Glycogen Assay Kit
    See all Glycogen kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue
  • Assay type
  • Sensitivity
    > 0.4 µg/ml
  • Range
    0.4 µg/ml - 2000 µg/ml
  • Assay time
    0h 40m
  • Product overview

    Glycogen Assay Kit (ab65620) is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.

    If your sample is likely to contain reducing substances, we recommend using Glycogen Assay Kit II (ab169558), as reducing substances may interfere with the assay detection method.

    Visit our FAQs page for tips and troubleshooting.

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in alpha-1,4 linkage, with branching via alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.



  • Total glycogen levels in C576bL6 mice astrocytes were determined by using Glycogen assay kit (ab65620). At 24 hours following OGD-reoxygenation, astrocytes had less glycogen levels compared to normoxia control.  Astrocytes treated with Methylene blue (MB) showed a higher glycogen content compared to non-MB treated, OGD-reoxygenation astrocytes. * p < 0.05; ## p < 0.001 Vs. OGD-reoxygenation control / 0 μM MB.

  • Example of fluorometric standard curve using Glycogen Assay Kit (ab65620).

  • Measurement of glycogen in various mouse tissues using Glycogen Assay Kit (ab65620).

  • Glycogen concentration measured in MBA-MB-231 cells (human breast adenocarcinoma cell line). 106 cells were prepared following protocol instructions, and several dilutions were measured using fluorometric detection.



This product has been referenced in:
  • Sobieski C  et al. Ambient but not local lactate underlies neuronal tolerance to prolonged glucose deprivation. PLoS One 13:e0195520 (2018). Read more (PubMed: 29617444) »
  • Guo J  et al. Liraglutide reduces hepatic glucolipotoxicity-induced liver cell apoptosis through NRF2 signaling in Zucker diabetic fatty rats. Mol Med Rep 17:8316-8324 (2018). Read more (PubMed: 29693190) »

See all 19 Publications for this product

Customer reviews and Q&As

Cells within collagen or Matrigel matrix are not ideal since when you homogenize the samples in the matrix and boil them, the matrix or components of the matrix can come along with the sample. This can affect the assay. Without knowing exactly what goe...

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If you are using something like Matrigel for culturing the primary hepatocytes, then it is essential to remove the gel and collect the cells before proceeding with the homogenization and boiling to extract the glycogen. Liver cells store glycogen and h...

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The fluorescent signal is quite stable for several hours when protected from light. However, we recommend measuring the plate immediately after incubation for the best results.

ab65620, Glycogen Assay Kit, can detect glycogen 0.0004 to 2 mg/ml. This kit can be used in both colorimetric and fluorometric applications. In this assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a...

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For 100-150mg of a tough tissue like muscle, I would definitely recommend using the KOH method. You can also consult the literature for any other glycogen extraction method that may be preferable.

I can confirm that glucose generates background readings. Since glucose is present in your sample, we strongly recommend to do a glucose control without the addition of hydrolysis enzyme to determine the level of glucose background in your sa...

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This kit was optimized using water, and unfortunately we don't have any data on whether Levamisol might work. This would have to be tested.

I hope this information is helpful to you. Please do not hesi...

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PBS can be used in place of water for homgenizing.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Are you inquiring about ab65620? If so, you will not need to use any lysis buffers with this product. For tissue or cell samples with this kit you will homogenize 10^6 cells or 10 mg tissue with 200 μl dH₂O on ice. Then boil the homogenates for 5 mi...

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The shipping temperature for this kit is +4C and for storage temperature please refer to the protocol.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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1-10 of 45 Abreviews or Q&A


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