Key features and details
- Rabbit polyclonal to Glycogen synthase 1/GYS1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glycogen synthase 1/GYS1 antibody
See all Glycogen synthase 1/GYS1 primary antibodies
DescriptionRabbit polyclonal to Glycogen synthase 1/GYS1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Glycogen synthase 1/GYS1.
Database link: P13807
- IHC-P: Human heart tissue. ICC/IF: HeLa cells. WB: HeLa, HEK-293T, BT-474 and mouse heart extract.
This product was previously labelled as Glycogen synthase 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab230422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/20000. Predicted molecular weight: 84 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionTransfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
PathwayGlycan biosynthesis; glycogen biosynthesis.
Involvement in diseaseDefects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
Sequence similaritiesBelongs to the glycosyltransferase 3 family.
- Information by UniProt
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
All lanes : Anti-Glycogen synthase 1/GYS1 antibody (ab230422) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell extracts
Lane 3 : BT-474 (Human ductal breast epithelial tumor cell line) cell extracts
Lane 4 : Mouse heart extracts
Developed using the ECL technique.
Predicted band size: 84 kDa
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Glycogen synthase 1/GYS1 using ab230422 (green) at a dilution of 1/50 in ICC/IF.
Nuclear counterstain: DAPI (blue).
Formalin-fixed, paraffin-embedded human heart tissue stained for Glycogen synthase 1/GYS1 with ab230422 at a 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230422 has not yet been referenced specifically in any publications.