Product nameAnti-Glycogen synthase 1/GYS1 antibody [EP817Y]
See all Glycogen synthase 1/GYS1 primary antibodies
DescriptionRabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Glycogen synthase 1/GYS1 aa 700-800 (C terminal). The exact sequence is proprietary.
- HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (Alexa Fluor® 488) (ab202759)
- Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (Alexa Fluor® 647) (ab202761)
- Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (Phycoerythrin) (ab210583)
- Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
Our Abpromise guarantee covers the use of ab40810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/50 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTransfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
PathwayGlycan biosynthesis; glycogen biosynthesis.
Involvement in diseaseDefects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
Sequence similaritiesBelongs to the glycosyltransferase 3 family.
- Information by UniProt
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40810, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab40810 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1/GYS1 RabMAb.
This product has been referenced in:
- Barker RG et al. Elevated GLUT4 and glycogenin protein abundance correspond to increased glycogen content in the soleus muscle of mdx mice with no benefit associated with taurine supplementation. Physiol Rep 6:N/A (2018). Read more (PubMed: 29484837) »
- Zhao Y et al. Decreased Glycogen Content Might Contribute to Chronic Stress-Induced Atrophy of Hippocampal Astrocyte volume and Depression-like Behavior in Rats. Sci Rep 7:43192 (2017). Read more (PubMed: 28233800) »