Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)


  • Product name

    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]
    See all Glycogen synthase 1/GYS1 primary antibodies
  • Description

    Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glycogen synthase 1/GYS1 aa 700-800 (C terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

     This product was previously labelled as Glycogen synthase 1


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab40810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/50 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.


  • Function

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • Pathway

    Glycan biosynthesis; glycogen biosynthesis.
  • Involvement in disease

    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • Sequence similarities

    Belongs to the glycosyltransferase 3 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all


  • Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 81 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?

  • Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40810, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab40810 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1/GYS1 RabMAb.


This product has been referenced in:

  • Barker RG  et al. Elevated GLUT4 and glycogenin protein abundance correspond to increased glycogen content in the soleus muscle of mdx mice with no benefit associated with taurine supplementation. Physiol Rep 6:N/A (2018). Read more (PubMed: 29484837) »
  • Kakhlon O  et al. Guaiacol as a drug candidate for treating adult polyglucosan body disease. JCI Insight 3:N/A (2018). Read more (PubMed: 30185673) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (MDA-MB-231 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
Loading amount
50 µg
MDA-MB-231 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 06 2018

Immunocytochemistry/ Immunofluorescence
Human Cell (A549 Lung cancer cells)
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
A549 Lung cancer cells
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Dimitra Kalamida

Verified customer

Submitted Mar 04 2015

Western blot
Mouse Tissue lysate - whole (Adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Loading amount
14 µg
Adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 21 2013


The immunogen sequence is within the last 25 amino acids of the C terminus of human GYS1. Yes, a single amino acid difference within the sequence that defines the epitope will be enough to reduce binding affinity, often to the point where there is no specific recognition at all. On the other hand, some subsitutions have no effect at all.

Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Placentas)
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Buffered Formalin

Abcam user community

Verified customer

Submitted Sep 05 2008

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