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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (15)

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Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
  • Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
  • Flow Cytometry - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
  • Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]
    See all Glycogen synthase 1/GYS1 primary antibodies
  • Description

    Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Glycogen synthase 1/GYS1 aa 700-800 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa whole cell lysate (ab150035), HEK293 cell lysate. IF: HeLa cells. Flow Cyt: HEK293
  • General notes

     This product was previously labelled as Glycogen synthase 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP817Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)
    • Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human GYS1 (Glycogen synthase 1) knockout HeLa cell line (ab265388)
  • KO cell lysates

    • Human GYS1 (Glycogen synthase 1) knockout HeLa cell lysate (ab257462)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Glycogen synthase 1/GYS1 protein (ab158615)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
ICC/IF (1)
1/250 - 1/500.
Flow Cyt
1/50 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
ICC/IF
1/250 - 1/500.
Flow Cyt
1/50 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • Pathway

    Glycan biosynthesis; glycogen biosynthesis.
  • Involvement in disease

    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • Sequence similarities

    Belongs to the glycosyltransferase 3 family.
  • Target information above from: UniProt accession P13807 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2997 Human
    • Entrez Gene: 14936 Mouse
    • Entrez Gene: 690987 Rat
    • Omim: 138570 Human
    • SwissProt: P13807 Human
    • SwissProt: Q9Z1E4 Mouse
    • SwissProt: A2RRU1 Rat
    • Unigene: 386225 Human
    • Unigene: 275654 Mouse
    • Unigene: 95278 Rat
    see all
  • Alternative names

    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

Images

  • Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    All lanes : Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/10000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : Glycogen synthase 1/GYS1 knockout HeLa lysate
    Lane 3 : HEK-293 lysate
    Lane 4 : Daudi lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 81 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab40810 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab40810 Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] was shown to specifically react with Glycogen synthase 1/GYS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265388 (knockout cell lysate ab257462) was used. Wild-type and Glycogen synthase 1/GYS1 knockout samples were subjected to SDS-PAGE. ab40810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 81 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Flow Cytometry - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40810, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)

    ab40810 at a 1:250 dilution staining HeLa cells using anti-Glycogen Synthase 1/GYS1 RabMAb.

  • Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (15)

Publishing research using ab40810? Please let us know so that we can cite the reference in this datasheet.

ab40810 has been referenced in 15 publications.

  • Shen J  et al. Pressure suppresses hepatocellular glycogen synthesis through activating the p53/Pten pathway. Mol Med Rep 19:5105-5114 (2019). PubMed: 31059076
  • Kakhlon O  et al. Guaiacol as a drug candidate for treating adult polyglucosan body disease. JCI Insight 3:N/A (2018). PubMed: 30185673
  • Rubio-Villena C  et al. Astrocytes: new players in progressive myoclonus epilepsy of Lafora type. Hum Mol Genet 27:1290-1300 (2018). PubMed: 29408991
  • Zang Y  et al. Improvement of Lipid and Glucose Metabolism by Capsiate in Palmitic Acid-Treated HepG2 Cells via Activation of the AMPK/SIRT1 Signaling Pathway. J Agric Food Chem 66:6772-6781 (2018). PubMed: 29886733
  • Barker RG  et al. Elevated GLUT4 and glycogenin protein abundance correspond to increased glycogen content in the soleus muscle of mdx mice with no benefit associated with taurine supplementation. Physiol Rep 6:N/A (2018). PubMed: 29484837
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Western blot abreview for Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U87 glioblastoma cell line)
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
30 µg
Treatment
FLAVOPIRIDOL
Specification
U87 glioblastoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Dr. Michele D'angelo

Verified customer

Submitted Feb 03 2020

Western blot abreview for Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
Loading amount
50 µg
Specification
MDA-MB-231 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jul 06 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549 Lung cancer cells)
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Specification
A549 Lung cancer cells
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Dr. Dimitra Kalamida

Verified customer

Submitted Mar 04 2015

Western blot abreview for Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Loading amount
14 µg
Specification
Adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 21 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Placentas)
Antigen retrieval step
Heat mediated
Specification
Placentas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Buffered Formalin
Read More

Abcam user community

Verified customer

Submitted Sep 05 2008

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