Recombinant
RabMAb

Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (Phycoerythrin) (ab210583)

Overview

  • Product name

    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (Phycoerythrin)
    See all Glycogen synthase 1/GYS1 primary antibodies
  • Description

    Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    within Human Glycogen synthase 1/GYS1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P13807

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as Glycogen synthase 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab210583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/50.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • Pathway

    Glycan biosynthesis; glycogen biosynthesis.
  • Involvement in disease

    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • Sequence similarities

    Belongs to the glycosyltransferase 3 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab210583 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210583, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • ab210583 staining Glycogen synthase 1/GYS1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210583 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

References

ab210583 has not yet been referenced specifically in any publications.

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