Anti-Glycogen synthase 2 antibody (ab83550)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Glycogen synthase 2 antibody
See all Glycogen synthase 2 primary antibodies -
Description
Rabbit polyclonal to Glycogen synthase 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish -
Immunogen
Synthetic peptide: TLSRAFPDKF HVELTSPPTT EGFKYPRPSS VPPSPSGSQA SSPQSSDVED , corresponding to a region within internal sequence amino acids 612 - 661 of Human Glycogen synthase 2 (NP_068776)
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Positive control
- Fetal muscle lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | Use a concentration of 5 µg/ml. | |
ELISA | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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Function
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
Pathway
Glycan biosynthesis; glycogen biosynthesis. -
Involvement in disease
Defects in GYS2 are the cause of glycogen storage disease type 0 (GSD0) [MIM:240600]; A metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood, high blood ketones and low alanine and lactate concentrations. Although feeding relieves symptoms, it often results in postprandial hyperglycemia and hyperlactatemia. -
Sequence similarities
Belongs to the glycosyltransferase 3 family. -
Post-translational
modificationsPrimed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme. - Information by UniProt
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Database links
- Entrez Gene: 2998 Human
- Entrez Gene: 232493 Mouse
- Entrez Gene: 25623 Rat
- Omim: 138571 Human
- SwissProt: P54840 Human
- SwissProt: Q8VCB3 Mouse
- SwissProt: P17625 Rat
- Unigene: 82614 Human
see all -
Alternative names
- EC 2.4.1.11 antibody
- Glycogen [starch] synthase antibody
- Glycogen starch synthase liver antibody
see all
Images
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Anti-Glycogen synthase 2 antibody (ab83550) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDa
Gel concentration 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 2 antibody (ab83550)IHC image of ab83550 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83550, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References
ab83550 has not yet been referenced specifically in any publications.