Anti-Glycogen synthase 2 antibody (ab83550)


  • Product name
    Anti-Glycogen synthase 2 antibody
    See all Glycogen synthase 2 primary antibodies
  • Description
    Rabbit polyclonal to Glycogen synthase 2
  • Host species
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: TLSRAFPDKF HVELTSPPTT EGFKYPRPSS VPPSPSGSQA SSPQSSDVED , corresponding to a region within internal sequence amino acids 612 - 661 of Human Glycogen synthase 2 (NP_068776)

  • Positive control
    • Fetal muscle lysate



Our Abpromise guarantee covers the use of ab83550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • Pathway
    Glycan biosynthesis; glycogen biosynthesis.
  • Involvement in disease
    Defects in GYS2 are the cause of glycogen storage disease type 0 (GSD0) [MIM:240600]; A metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood, high blood ketones and low alanine and lactate concentrations. Although feeding relieves symptoms, it often results in postprandial hyperglycemia and hyperlactatemia.
  • Sequence similarities
    Belongs to the glycosyltransferase 3 family.
  • Post-translational
    Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • Glycogen [starch] synthase antibody
    • Glycogen starch synthase liver antibody
    • Glycogen synthase 2 liver antibody
    • Gys2 antibody
    • GYS2_HUMAN antibody
    • liver antibody
    see all


  • Anti-Glycogen synthase 2 antibody (ab83550) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

    Gel concentration 12%
  • IHC image of ab83550 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83550, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab83550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thanks for your call yesterday and for your patience while I have been in touch with the lab about ab83550. I've attached a lab image showing Western blot results in human liver samples.

We discussed our testing discount program, since we hav...

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