Key features and details
- Rabbit polyclonal to Glycogenin 1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glycogenin 1 antibody
DescriptionRabbit polyclonal to Glycogenin 1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- ICC/IF: U-251 MG. WB: Human cell line RT4. IHC-P: Human testis tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionSelf-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.
PathwayGlycan biosynthesis; glycogen biosynthesis.
Involvement in diseaseDefects in GYG1 are the cause of glycogen storage disease type 15 (GSD15) [MIM:613507]. It is a metabolic disorder resulting in muscle weakness, associated with the glycogen depletion in skeletal muscle, and cardiac arrhythmia, associated with the accumulation of abnormal storage material in the heart. The skeletal muscle shows a marked predominance of slow-twitch, oxidative muscle fibers and mitochondrial proliferation.
Sequence similaritiesBelongs to the glycogenin family.
modificationsSelf-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195.
- Information by UniProt
- Glycogenin antibody
- Glycogenin-1 antibody
- Glycogenin1 antibody
Lane 2 : Anti-Glycogenin 1 antibody (ab272606) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Human cell line RT4
Paraffin-embedded formalin-fixed Human testis tissue stained for Glycogenin 1 using ab272606 at 1/500 in immunohistochemical analysis.
PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling Glycogenin 1 (green) using ab272606at 2 ug/ml in ICC/IF analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272606 has not yet been referenced specifically in any publications.