Overview

  • Product name

    Glycolysis Stress Test Companion Assay
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Glycolysis Stress Test Companion Assay (ab222945) allows glycolytic characterization at user-defined glucose concentrations in live cells when used in combination with our Glycolysis Assay [Extracellular Acidification] (ab197244)


    The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.

  • Notes

    Learn more about the full range of assays to measure glycolysis, oxygen consumption, fatty acid oxidation and metabolic flux in live cells.

    Or review the full metabolism assay guide for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 55 tests
    2-DG 1 x 600µl
    Glucose 1 vial
    Glucose Oxidase Signal Control 1 vial
    Glucose-free Respiration Buffer 1 tablet
    Oligomycin 1 vial

Images

Protocols

References

ab222945 has not yet been referenced specifically in any publications.

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