Glycolysis Stress Test Complete Assay Kit (ab222946)

Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. This product combines Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945) in one practical kit for single purchase.

The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.