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Glycolysis Stress Test Complete Assay Kit (ab222946)

  • Datasheet
  • SDS
  • Protocol Booklet
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Extracellular acidification of A549 cells treated with 2-DG (purple) and oligomycin (black).
  • Glycolytic characterization of A549 cells.
  • Increased compensatory glycolysis upon increased ATP demand in monensin-treated HepG2 cells.

Key features and details

  • Assay type: Cell-based (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Adherent cells

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Overview

  • Product name

    Glycolysis Stress Test Complete Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. This product combines Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945) in one practical kit for single purchase.


    The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.

  • Notes

    Learn more about the full range of assays to measure glycolysis, oxygen consumption, fatty acid oxidation and metabolic flux in live cells.

    Or review the full metabolism assay guide for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 55 tests
    2-DG 1 x 600µl
    Glucose 1 vial
    Glucose Oxidase Signal Control 1 vial
    Glucose-free Respiration Buffer 1 tablet
    Glycolysis Assay Reagent (lyophilized) 1 vial
    Oligomycin 1 vial
    Respiration Buffer 1 tablet

Associated products

  • Related Products

    • Glycolysis Assay [Extracellular acidification] (ab197244)
    • Glycolysis Stress Test Companion Assay (ab222945)

Images

  • Extracellular acidification of A549 cells treated with 2-DG (purple) and oligomycin (black).
    Extracellular acidification of A549 cells treated with 2-DG (purple) and oligomycin (black).
  • Glycolytic characterization of A549 cells.
    Glycolytic characterization of A549 cells.
  • Increased compensatory glycolysis upon increased ATP demand in monensin-treated HepG2 cells.
    Increased compensatory glycolysis upon increased ATP demand in monensin-treated HepG2 cells.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab222946? Please let us know so that we can cite the reference in this datasheet.

    ab222946 has not yet been referenced specifically in any publications.

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