Anti-Glycophorin A antibody (ab40844)
Key features and details
- Goat polyclonal to Glycophorin A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glycophorin A antibody
See all Glycophorin A primary antibodies -
Description
Goat polyclonal to Glycophorin A -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
SVEIENPETSDQ
, corresponding to C terminal amino acids 139-150 of Human Glycophorin A -
Positive control
- WB: Human peripheral blood monocytes and kidney lysates. Recombinant Human Glycophorin A protein (ab114330).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
1 hour primary incubation is recommended for this product. The observed molecular weight differs from the predicted molecular weight due to the formation of dimers as described in the literature (PMID: 8204624). |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa). 1 hour primary incubation is recommended for this product. The observed molecular weight differs from the predicted molecular weight due to the formation of dimers as described in the literature (PMID: 8204624). |
Target
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Function
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). -
Sequence similarities
Belongs to the glycophorin A family. -
Post-translational
modificationsThe major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). -
Cellular localization
Cell membrane. Appears to be colocalized with SLC4A1. - Information by UniProt
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Database links
- Entrez Gene: 2993 Human
- Omim: 111300 Human
- SwissProt: P02724 Human
- Unigene: 434973 Human
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Alternative names
- AI853584 antibody
- Blood group--MN locus antibody
- CD_antigen=CD235a antibody
see all
Images
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All lanes : Anti-Glycophorin A antibody (ab40844) at 1 µg/ml
Lane 1 : Human peripheral blood monocyte lysate
Lane 2 : Human kidney lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 16 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
The observed molecular weight differs from the predicted molecular weight due to the formation of dimers as described in the literature (PMID: 8204624).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab40844 has not yet been referenced specifically in any publications.