Recombinant
RabMAb

Recombinant Anti-Glycophorin A antibody [EPR8199] (ab134111)

Overview

  • Product name

    Anti-Glycophorin A antibody [EPR8199]
    See all Glycophorin A primary antibodies
  • Description

    Rabbit monoclonal [EPR8199] to Glycophorin A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human Glycophorin A.

  • Positive control

    • Human fetal liver lysate, Human lung tissue, Human spleen tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
    • Sequence similarities

      Belongs to the glycophorin A family.
    • Post-translational
      modifications

      The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
    • Cellular localization

      Cell membrane. Appears to be colocalized with SLC4A1.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI853584 antibody
      • Blood group--MN locus antibody
      • CD_antigen=CD235a antibody
      • CD235a antibody
      • GLPA_HUMAN antibody
      • Glycophorin A (MNS blood group) antibody
      • Glycophorin A antibody
      • Glycophorin A, included antibody
      • Glycophorin-A antibody
      • GlycophorinA antibody
      • GPA antibody
      • GPErik antibody
      • GpMiIII antibody
      • GPSAT antibody
      • GYPA antibody
      • GYPA, included antibody
      • HGpMiIII antibody
      • HGpMiV antibody
      • HGpMiX antibody
      • HGpMiXI antibody
      • HGpSta(C) antibody
      • MN antibody
      • MN sialoglycoprotein antibody
      • MNS antibody
      • PAS-2 antibody
      • PAS2 antibody
      • Sialoglycoprotein alpha antibody
      see all

    Images

    • Anti-Glycophorin A antibody [EPR8199] (ab134111) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 38 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Glycophorin A with ab134111 at 1/100.
    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Glycophorin A with ab134111 at 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134111 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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