Overview

  • Product name

    Anti-Glycophorin A antibody [JC159] (FITC)
    See all Glycophorin A primary antibodies
  • Description

    Mouse monoclonal [JC159] to Glycophorin A (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Glycophorin A. Membrane preparation from splenic hairy cell leukemia.

  • Positive control

    • Flow Cytometry: Human peripheral blood cells (erythrocytes and leukocytes).

Properties

Applications

Our Abpromise guarantee covers the use of ab233580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

Target

  • Function

    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • Sequence similarities

    Belongs to the glycophorin A family.
  • Post-translational
    modifications

    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • Cellular localization

    Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all

Images

  • Surface staining of Glycophorin A (CD235a) in human peripheral blood cells (erythrocytes and leukocytes) with ab233580. Gated on leukocytes and erythrocytes.

References

ab233580 has not yet been referenced specifically in any publications.

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